Nuclear Expression of GS28 Protein: A Novel Biomarker that Predicts Prognosis in Colorectal Cancers

Autor: Su Young Kim, Ahwon Lee, Do Eun Rim, Oh-Joo Kwon, Hyung Jae Yoo, Seong-Whan Jeong, Yinji Cui, Eun Sun Jung, Jun Gi Kim, Sung Hak Lee, Seung Taek Oh
Rok vydání: 2017
Předmět:
0301 basic medicine
Oncology
Adult
Male
medicine.medical_specialty
Stromal cell
Colorectal cancer
Perineural invasion
Golgi Apparatus
Kaplan-Meier Estimate
Endoplasmic Reticulum
Disease-Free Survival
03 medical and health sciences
0302 clinical medicine
Biologic Marker
Internal medicine
GS28 protein
medicine
Biomarkers
Tumor
Humans
Epidermal growth factor receptor
Neoplasm Metastasis
Aged
Biologic marker
Aged
80 and over
biology
business.industry
Biological Transport
General Medicine
Middle Aged
Qb-SNARE Proteins
medicine.disease
Prognosis
SNARE proteins
Colorectal Carcinoma
ErbB Receptors
Gene Expression Regulation
Neoplastic
030104 developmental biology
030220 oncology & carcinogenesis
Golgi Complex
biology.protein
Immunohistochemistry
Biomarker (medicine)
Female
business
Protein A
Colorectal Neoplasms
Research Paper
Zdroj: International Journal of Medical Sciences
ISSN: 1449-1907
Popis: Aims: GS28 (Golgi SNARE protein, 28 kDa), a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) protein family, plays a critical role in mammalian endoplasmic reticulum (ER)-Golgi or intra-Golgi vesicle transport. To date, few researches on the GS28 protein in human cancer tissues have been reported. In this study, we assessed the prognostic value of GS28 in patients with colorectal cancer (CRC). Methods and results: We screened for GS28 expression using immunohistochemistry in 230 surgical CRC specimens. The CRCs were right-sided and left-sided in 28.3% (65/230) and 71.3% (164/230) of patients, respectively. GS28 staining results were available in 214 cases. Among these, there were 26 nuclear predominant cases and 188 non-nuclear predominant cases. Stromal GS28 expression was noted in 152 cases of CRC. GS28 nuclear predominant immunoreactivity was significantly associated with advanced tumour stage (p = 0.045) and marginally associated with perineural invasion (p = 0.064). Decreased GS28 expression in the stromal cells was significantly associated with lymph node metastasis (N stage; p = 0.036). GS28 expression was not associated with epidermal growth factor receptor (EGFR) immunohistochemical positivity or KRAS mutation status. Investigation of the prognostic value of GS28 with Kaplan-Meier analysis revealed a correlation with overall survival (p = 0.004). Cases with GS28 nuclear predominant expression had significantly poorer overall survival than those with a non-nuclear predominant pattern. Conclusions: Taken together, these results indicate that GS28 nuclear predominant expression could serve as a prognostic marker for CRC and may help in identifying aggressive forms of CRC.
Databáze: OpenAIRE
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