Nuclear localization of HTLV-I bZIP factor (HBZ) is mediated by three distinct motifs
| Autor: | Patrick Hivin, Mélissa Frédéric, Charlotte Arpin-André, Jihane Basbous, Bernard Gay, Sabine Thébault, Jean-Michel Mesnard |
|---|---|
| Přispěvatelé: | Mesnard, Jean-Michel, Infections rétrovirales et signalisation cellulaire (IRSC), Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de génétique des maladies rares. Pathologie moleculaire, etudes fonctionnelles et banque de données génétiques (LGMR), Université Montpellier 1 (UM1)-IFR3, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), ARC, Ligue contre le Cancer, IFR3, Université Montpellier 1 (UM1)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2005 |
| Předmět: |
Transcription
Genetic Nucleolus Proto-Oncogene Proteins c-jun Amino Acid Motifs Nuclear Localization Signals Retroviridae Proteins MESH: Nuclear Localization Signals MESH: Amino Acid Motifs Transcription (biology) Heterochromatin Chlorocebus aethiops MESH: Animals [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology 0303 health sciences Human T-lymphotropic virus 1 030302 biochemistry & molecular biology MESH: Transcription Factors Immunohistochemistry 3. Good health MESH: COS Cells MESH: Heterochromatin Basic-Leucine Zipper Transcription Factors [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology COS Cells trafic nucléaire MESH: Cell Nucleus MESH: Mutation [SDV.CAN]Life Sciences [q-bio]/Cancer MESH: Microscopy Electron Biology MESH: Basic-Leucine Zipper Transcription Factors 03 medical and health sciences Splicing factor Viral Proteins [SDV.CAN] Life Sciences [q-bio]/Cancer Animals Humans MESH: Cell Nucleus Structures leucémie Transcription factor 030304 developmental biology Cell Nucleus Nucleoplasm MESH: Human T-lymphotropic virus 1 MESH: Humans MESH: Proto-Oncogene Proteins c-jun MESH: Transcription Genetic MESH: Immunohistochemistry Cell Biology Molecular biology MESH: Cercopithecus aethiops MESH: Viral Proteins Cell Nucleus Structures Renal disorders [UMCN 5.4] Microscopy Electron Cajal body HTLV-1 Mutation Nuclear localization sequence Transcription Factors |
| Zdroj: | Journal of Cell Science, 118, Pt 7, pp. 1355-62 Journal of Cell Science, 118, 1355-62 Journal of Cell Science Journal of Cell Science, 2005, 118 (Pt 7), pp.1355-62. ⟨10.1242/jcs.01727⟩ Journal of Cell Science, Company of Biologists, 2005, 118 (Pt 7), pp.1355-62. ⟨10.1242/jcs.01727⟩ |
| ISSN: | 0021-9533 1477-9137 |
| DOI: | 10.1242/jcs.01727⟩ |
| Popis: | Contains fulltext : 48999thebault.pdf (Publisher’s version ) (Open Access) The genome of the human T-cell leukemia virus type I (HTLV-I) codes for a basic leucine zipper protein, HBZ, capable of repressing JUN activity and viral transcription. Transient expression in mammalian cells showed that HBZ was targeted to the nucleus, where it accumulated in nuclear speckles. By using a complementary set of deletion mutants, we report here that the nuclear targeting of HBZ is mediated by three distinct nuclear localization signals and that at least two are necessary for the translocation of HBZ to the nucleus. Moreover, the resulting mutant proteins distribute throughout the nucleoplasm and/or into the nucleoli, whereas the wild-type HBZ exclusively accumulates in nuclear speckles, suggesting that the integrity of the protein is required for its speckle localization. We also demonstrate that the HBZ-containing speckles do not correspond to Cajal bodies, splicing factor compartments, or promyelocytic leukemia oncoprotein bodies. Unexpectedly, by using immunogold electron microscopy, we found HBZ localized to heterochromatin. Until now, such characteristics had never been described for a transcription factor and could explain the inhibitory activity of HBZ. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|