Nerve-tumour interaction enhances the aggressiveness of oral squamous cell carcinoma
| Autor: | Tsung Lun Lee, Yi Fen Wang, Wing Yin Li, Pei Yin Wei, Shyh Kuan Tai, Po Hsien Chiu, Pen Yuan Chu, Muh Hwa Yang |
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| Rok vydání: | 2018 |
| Předmět: |
Cell type
Perineural invasion Mice Nude Pathogenesis 03 medical and health sciences Paracrine signalling 0302 clinical medicine Gentamicin protection assay In vivo Cell Movement Cell Line Tumor Medicine Animals Humans Neoplasm Invasiveness Peripheral Nerves 030223 otorhinolaryngology Retrospective Studies business.industry Soft tissue Prognosis Peripheral stomatognathic diseases Otorhinolaryngology 030220 oncology & carcinogenesis Lymphatic Metastasis Cancer research Carcinoma Squamous Cell Mouth Neoplasms business |
| Zdroj: | Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-LaryngologyCervico-Facial SurgeryREFERENCES. 44(6) |
| ISSN: | 1749-4486 |
| Popis: | Objectives Perineural invasion (PNI) is a poor prognostic pathologic feature of oral squamous cell carcinoma (OSCC). The mechanisms of PNI remain poorly understood, and nerve-tumour interactions have been implicated for its pathogenesis. Design and setting Systematic investigation of nerve-tumour interactions was performed using fresh human peripheral nerve. In vitro and in vivo models were used to determine the ability of human peripheral nerves to enhance OSCC migration/invasion. Retrospective cohort study was also carried out in one medical centre from 2001 to 2009. Participants 314 T1-2 OSCC patients. Main outcome measures In the transwell migration/invasion assay, the cells in five representative fields were counted. In the nerve implantation model, tumour size was estimated. PNI quantification by PNI focus number was carried out in the OSCC patients to correlate with cervical lymph node metastasis and oncologic outcomes. Results The transwell migration/invasion assay demonstrated that human peripheral nerves, compared with subcutaneous soft tissue, significantly enhanced the migration/invasion abilities of OSCC. Moreover, the enhanced migration was dose-dependent with increased length or number of peripheral nerve segments. The nerve implantation model showed that human peripheral nerve also enhanced OSCC growth in vivo. Finally, increased PNI focus number was found dose-dependently associated with increased cervical lymph node metastasis and decreased 5-year disease-specific survival rates. Conclusions These results clearly indicated the presence of nerve-tumour interaction that involved paracrine influences leading to aggressiveness of OSCC. Further investigations are required to explore key cell types and molecules involved in nerve-tumour interactions for future therapeutic targeting of PNI in OSCC. |
| Databáze: | OpenAIRE |
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