Stabilization of Chlorophyll a-binding Apoproteins P700, CP47, CP43, D2, and D1 by Chlorophyll a or Zn-pheophytin a
| Autor: | Michael Helfrich, Lutz A. Eichacker, Bernd Müller, Wolfhart Rüdiger |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chlorophyll
Pheophytin Chlorophyll a Photosynthetic Reaction Center Complex Proteins Light-Harvesting Protein Complexes Chlorophyll c Biochemistry Pigment chemistry.chemical_compound Magnesium Molecular Biology P700 Chlorophyll A Pheophytins Photosystem II Protein Complex Cell Biology De novo synthesis Zinc chemistry visual_art visual_art.visual_art_medium Biophysics Etioplasts Apoproteins |
| Zdroj: | Journal of Biological Chemistry. 271:32174-32179 |
| ISSN: | 0021-9258 |
| Popis: | Stabilization of chlorophyll a-binding apoproteins P700, CP47, CP43, D2, and D1 against proteolytic degradation has been investigated through in vitro synthesis of chlorophyll a or Zn-pheophytin a in intact etioplasts from barley. Stabilization of the apoproteins was dependent on the concentration of chlorophyll a or Zn-pheophytin a. Zn-pheophytin a was superior to chlorophyll a with respect to the concentration of pigment required for an equal yield of the stabilized chlorophyll a protein CP47, CP43, and P700 and for the total yield of chlorophyll a proteins. Zn-pheophytin a was most efficient for stabilizing CP47 and, at an increased concentration, efficient for stabilizing CP43, P700, and D1. Stabilization of apoproteins was highest after de novo synthesis of 90-300 pmol of Zn-pheophytin a or of about 400-600 pmol of chlorophyll a/4.2 x 10(7) etioplasts. The yield of stabilized chlorophyll proteins decreased at higher concentrations of Zn-pheophytin a, but was unaffected by higher concentrations of chlorophyll a. |
| Databáze: | OpenAIRE |
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