Immunohistochemical Analysis on Cortex-to-Cortex Healing After Mandibular Vertical Ramus Osteotomy: A Preliminary Study
| Autor: | Sang Yoon Kim, Young-Soo Jung, Han Sung Jung, Hyung-Sik Park, Hwi-Dong Jung |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty medicine.medical_treatment Osteotomy Sagittal Split Ramus Mandibular Osteotomy Osteotomy Bone morphogenetic protein 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Vascularity Dogs Transforming Growth Factor beta medicine Internal fixation Animals Wound Healing business.industry 030206 dentistry Anatomy Immunohistochemistry Up-Regulation Vascular endothelial growth factor 030104 developmental biology Otorhinolaryngology chemistry Bone Morphogenetic Proteins Cytokines Surgery Matrix Metalloproteinase 3 Oral Surgery medicine.symptom business Wound healing Transforming growth factor |
| Zdroj: | Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons. 76(2) |
| ISSN: | 1531-5053 |
| Popis: | Purpose The present study analyzed the expression of specific cytokines in the transforming growth factor (TGF)-β superfamily postoperatively after mandibular vertical ramus osteotomy (VRO). Materials and Methods Four beagle dogs were enrolled and euthanized at 1, 2, 4, and 8 weeks postoperatively for immunohistochemical analysis using 6 specific antibodies (bone morphogenetic protein [BMP]-2/4, BMP-7, TGF-β2, TGF-β3, matrix metalloproteinase-3, and vascular endothelial growth factor [VEGF]). The results from the surgical site and control (adjacent area) were compared. Results Generalized upregulation of BMP-2/4 was observed in all healing periods, and the strongest expression of BMP-7 was observed at 1 week postoperatively. The strongest expression of TGF-β2 was observed at 8 weeks with increasing pattern. The strong expression of TGF-β3 was observed at 1 and 4 weeks, with the strongest expression of VEGF at 1 week, with a decreasing pattern. No notable uptake was detected with the 6 specific antibodies in the adjacent bone (control). Conclusions The absence of internal fixation after VRO led to dynamic healing with a specific expression pattern of BMP-7 and TGF-β2. The anatomic factors, including sufficient preexisting vascularity, led to the earlier expression pattern of VEGF. |
| Databáze: | OpenAIRE |
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