Cholesterol Oxidase: A Potent Insecticidal Protein Active Against Boll Weevil Larvae
| Autor: | R.D. Sammons, J.T. Greenplate, S.R. Sims, M.G. Jennings, D.R. Corbin, R.J. Stonard, M. Tran, M.J. Prinsen, J.P. Purcell, J.C. Pershing, Jan S. Ryerse |
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| Rok vydání: | 1993 |
| Předmět: |
Insecticides
Cholesterol oxidase media_common.quotation_subject Molecular Sequence Data Bacillus thuringiensis Biophysics Insect Biochemistry Bacterial Proteins Animals Amino Acid Sequence Molecular Biology media_common chemistry.chemical_classification Gossypium Larva Sequence Homology Amino Acid biology fungi Midgut Cell Biology Sterol Esterase biology.organism_classification Bacillales Streptomyces Diet Coleoptera Enzyme chemistry Anthonomus |
| Zdroj: | Biochemical and Biophysical Research Communications. 196:1406-1413 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.2409 |
| Popis: | The discovery of proteins that control insects is critical for the continued growth of the agricultural biotechnology industry. A highly efficacious protein that killed boll weevil (Anthonomus grandis grandis Boheman) larvae was discovered in Streptomyces culture filtrates. The protein was identified as cholesterol oxidase (E.C 1.1.3.6). Purified cholesterol oxidase was active against boll weevil larvae at a concentration (LC50 = 20.9 μg/ml) comparable to the bioactivity of Bacillus thuringiensis proteins against other insect pests. Histological studies demonstrated that cholesterol oxidase lysed the boll weevil midgut epithelium, suggesting that this is the primary mechanism of lethality. |
| Databáze: | OpenAIRE |
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