Purification and Properties of the Klebsiella aerogenes UreE Metal-Binding Domain, a Functional Metallochaperone of Urease
| Autor: | Scott B. Mulrooney, Sarah K. Ward, Robert P. Hausinger |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Urease
Metal binding C-terminus Enterobacter aerogenes Biology biology.organism_classification Enzymes and Proteins Microbiology Protein Structure Tertiary Protein structure Plasmid Bacterial Proteins Biochemistry Metals Domain (ring theory) biology.protein Nickel ions Carrier Proteins Molecular Biology Molecular Chaperones Plasmids |
| Zdroj: | Journal of Bacteriology. 187:3581-3585 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Klebsiella aerogenes UreE, a metallochaperone that delivers nickel ions during urease activation, consists of distinct “peptide-binding” and “metal-binding” domains and a His-rich C terminus. Deletion analyses revealed that the metal-binding domain alone is sufficient to facilitate urease activation. This domain was purified and shown to exhibit metal-binding properties similar to those of UreE lacking only the His-rich tail. |
| Databáze: | OpenAIRE |
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