Effect of copper intoxication on rat liver proteasome activity: relationship with oxidative stress
| Autor: | Elina Tzvetanova, Marián Kukan, Lubomir Petrov, Albena Alexandrova, Mila Kessiova, Almira Georgieva, Margarita Kirkova |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Proteasome Endopeptidase Complex Health Toxicology and Mutagenesis Drinking Toxicology medicine.disease_cause Protein oxidation Biochemistry Models Biological Antioxidants Lipid peroxidation Superoxide dismutase chemistry.chemical_compound Internal medicine MG132 medicine Animals Rats Wistar Molecular Biology Glutathione Transferase chemistry.chemical_classification Reactive oxygen species Glutathione Peroxidase biology Superoxide Dismutase Copper toxicity Body Weight General Medicine Glutathione Feeding Behavior medicine.disease Catalase Rats Oxidative Stress Endocrinology chemistry Liver biology.protein Molecular Medicine Lipid Peroxidation Oxidative stress Copper |
| Zdroj: | Journal of biochemical and molecular toxicology. 22(5) |
| ISSN: | 1099-0461 |
| Popis: | Copper toxicity is associated with formation of reactive oxygen species, which are capable to oxidize proteins. The selective removal of the latter by the 20S proteasome is considered an essential part of the cell antioxidant defense system. The aim of the present study was to investigate whether peptidase activities of rat liver proteasomes were affected by chronic (40 mg CuSO(4)/rat/daily with the drinking water for 2 weeks) and acute (20 mg/kg CuSO(4), s.c.) copper treatment. To evaluate the role of proteasome, its inhibitor MG132 was also used. The degree of copper-induced oxidative stress (OS), established by measuring lipid peroxidation, protein oxidation, and cellular glutathione level, as well as activities of antioxidant enzymes--catalase, superoxide dismutase, and gultathionine peroxidase, depended on the mode of copper administration. Chronic copper administration (mild oxidative stress) did not affect proteasome activities, whereas acute copper treatment (severe oxidative stress) caused a decline in chymotryptic- and tryptic-like activities. The treatment of copper-loaded animals with MG132 did not change copper-induced alterations in the tested indices, except an additional increase in protein oxidation and inhibition of glutathionine peroxidase activity. The results suggested that the in vivo copper-induced oxidative stress was associated with changes in the catalytic activity of proteasome. |
| Databáze: | OpenAIRE |
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