Mucosal immunization with PLGA-microencapsulated DNA primes a SIV-specific CTL response revealed by boosting with cognate recombinant modified vaccinia virus Ankara
| Autor: | Tomáš Hanke, Stuart D. Dowall, Sally Sharpe, Mike Dennis, Andrew J. McMichael, Martin P. Cranage, Anne Margaret Tinsley-Bown |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Time Factors
Immunogen Lymphoid Tissue Polymers Drug Compounding Immunization Secondary Pilot Projects Vaccinia virus chemical and pharmacologic phenomena Microparticles Biology Multiepitope medicine.disease_cause Epitope Virus law.invention chemistry.chemical_compound Polylactic Acid-Polyglycolic Acid Copolymer Administration Rectal law Virology Vaccines DNA medicine Animals Cytotoxic T cell Lactic Acid Intestinal Mucosa AIDS Vaccines Drug Carriers Vaccination HIV DNA MVA Simian immunodeficiency virus Cytotoxicity Tests Immunologic Macaca mulatta CTL chemistry Immunology Recombinant DNA Simian Immunodeficiency Virus Vaccinia Vaccine Macaque Polyglycolic Acid T-Lymphocytes Cytotoxic |
| DOI: | 10.1016/s0042-6822(03)00282-4 |
| Popis: | Systemically administered DNA encoding a recombinant human immunodeficiency virus (HIV) derived immunogen effectively primes a cytotoxic T lymphocyte (CTL) response in macaques. In this further pilot study we have evaluated mucosal delivery of DNA as an alternative priming strategy. Plasmid DNA, pTH.HW, encoding a multi-CTL epitope gene, was incorporated into poly(d,l-lactic-co-glycolic acid) microparticles of less than 10 μm in diameter. Five intrarectal immunizations failed to stimulate a circulating vaccine-specific CTL response in 2 Mamu-A*01+ rhesus macaques. However, 1 week after intradermal immunization with a cognate modified vaccinia virus Ankara vaccine MVA.HW, CTL responses were detected in both animals that persisted until analysis postmortem, 12 weeks after the final boost. In contrast, a weaker and less durable response was seen in an animal vaccinated with the MVA construct alone. Analysis of lymphoid tissues revealed a disseminated CTL response in peripheral and regional lymph nodes but not the spleen of both mucosally primed animals. |
| Databáze: | OpenAIRE |
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