LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation

Autor: Xin Zhao, Zhaoji Su, Jie Lin, Ruxia Deng, Ping Wang, Xiansheng Lu, Xiaoyu Wang, Rui Cai, Chang Hong
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Proteomics
0301 basic medicine
Epithelial-Mesenchymal Transition
Esophageal Neoplasms
Carcinogenesis
lcsh:Medicine
Biology
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
Metastasis
03 medical and health sciences
0302 clinical medicine
Western blot
Cell Movement
Esophageal squamous cell carcinoma
Cell Line
Tumor
medicine
Humans
Neoplasm Invasiveness
Epithelial–mesenchymal transition
Fibroblast
neoplasms
Cancer-associated fibroblasts
Cell Proliferation
medicine.diagnostic_test
FN1
Research
Mesenchymal stem cell
lcsh:R
General Medicine
medicine.disease
digestive system diseases
Gene Expression Regulation
Neoplastic
030104 developmental biology
medicine.anatomical_structure
Latent TGF-beta Binding Proteins
Head and Neck Neoplasms
030220 oncology & carcinogenesis
LTBP1
Carcinoma
Squamous Cell
Cancer research
Immunohistochemistry
Transforming growth factor
Zdroj: Journal of Translational Medicine, Vol 18, Iss 1, Pp 1-13 (2020)
Journal of Translational Medicine
ISSN: 1479-5876
Popis: Background Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent cancers worldwide. Due to its high morbidity and mortality rates, it is urgent to find a molecular target that contributes to esophageal carcinogenesis and progression. In this research, we aimed to investigate the functions of Latent transforming growth factor β binding protein 1(LTBP1) in ESCC progression and elucidate the underlying mechanisms. Methods The tandem mass tag-based quantitative proteomic approach was applied to screen the differentially expressed proteins (DEPs) between 3 cases of ESCC tumor samples and paired normal tissues. Then the DEPs were validated in human ESCC tissues using western blot assays and GEPIA database respectively. The expression level of LTBP1 was detected in 152 cases of ESCC tissues and paired normal tissues. Loss-of-function assays were performed to detect the function of LTBP1 in vivo and in vitro. Immunofluorescence and Western blot assays were used to detect the expression of apoptosis, epithelial–mesenchymal transition (EMT) and cancer-associated fibroblasts (CAFs) markers. Results A total of 39 proteins were screened to be up-regulated (ratio > 2.0) in all three ESCC tissues. The results of immunohistochemistry assays indicated that the expression level of LTBP1 was higher in ESCC tissues than that in paired normal tissues (p Conclusion Overexpression of LTBP1 was associated with lymph node metastasis in ESCC. Our results indicated that LTBP1 not only increased the malignant behaviors of ESCC cells but also induced EMT and CAFs transformation. Our studies suggested an oncogenic role of LTBP1 in ESCC progression and it may serve as a potential therapeutic target for ESCC patients.
Databáze: OpenAIRE
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