Chemoselective ratiometric imaging of protein S-sulfenylation
| Autor: | W. A. Walker, John E. Crellin, Sarah L. Veatch, Paul M. Jenkins, Matthew B. Stone, Christopher T.M.B. Tom, Lyanne Gomez-Rodriguez, Jeannie L. Hernandez, Hashim F. Motiwala, Dahvid Davda, Kristin J. Labby, Brent R. Martin, Yu Hsuan Kuo |
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| Rok vydání: | 2017 |
| Předmět: |
Fluorophore
010405 organic chemistry Metals and Alloys General Chemistry 010402 general chemistry Photochemistry 01 natural sciences Fluorescence Article Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound chemistry Dimedone Materials Chemistry Ceramics and Composites Sulfenic acid |
| Zdroj: | Chemical Communications. 53:7385-7388 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c7cc02285a |
| Popis: | Here we report a ratiometric fluorescent probe for chemoselective conjugation to sulfenic acids in living cells. Our approach couples an α-fluoro-substituted dimedone to an aminonaphthalene fluorophore (F-DiNap), which upon sulfenic acid conjugation is locked as the 1,3-diketone, changing the fluorophore excitation. F-DiNap reacts with S-sulfenylated proteins at equivalent rates to current probes, but the α-fluorine substitution blocks side-reactions with biological aldehydes. |
| Databáze: | OpenAIRE |
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