CRNDE Contributes Cervical Cancer Progression by Regulating miR-4262/ZEB1 Axis
| Autor: | Qin-Xue Cao, Jun Tian, Shaoqin Yang, Lu Ren |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
cervical cancer miR-4262 medicine.medical_treatment OncoTargets and Therapy Targeted therapy 03 medical and health sciences 0302 clinical medicine CRNDE ZEB1 Medicine Pharmacology (medical) Viability assay Original Research Cervical cancer Oncogene business.industry Cell growth wnt/β-catenin pathway Wnt signaling pathway Cell migration medicine.disease 030104 developmental biology Oncology Apoptosis 030220 oncology & carcinogenesis Cancer research business |
| Zdroj: | OncoTargets and therapy |
| ISSN: | 1178-6930 |
| DOI: | 10.2147/ott.s263505 |
| Popis: | Lu Ren, Shaoqin Yang, Qinxue Cao, Jun Tian Department of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng, 475001 Henan, People’s Republic of ChinaCorrespondence: Jun TianDepartment of Obstetrics and Gynecology, Huaihe Hospital of Henan University, Kaifeng 475001, Henan, People’s Republic of ChinaTel +86 371-23906579Email dfwmex@163.comBackground: Cervical cancer is a lethal gynecologic cancer in women. Long non-coding RNA colorectal neoplasia differentially expressed (LncRNA CRNDE) was recognized as a significant oncogene in multiple cancers. However, the functional role of CRNDE in cervical cancer remains poorly explored.Methods: The expression of CRNDE, microRNA-4262 (miR-4262) and zinc-finger E-box binding homeobox 1 (ZEB1) in cervical cancer tumors and cells was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Colony formation and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) were performed to detect cell viability. Flow cytometry and caspase-3 activity assay were conducted to evaluate cell apoptosis. The interaction between miR-4262 and CRNDE or ZEB1 was verified by dual-luciferase reporter system. Transwell assay was employed to evaluate cell migration and invasion. The relative protein expression was assessed by Western blot.Results: CRNDE and ZEB1 were up-regulated, while miR-4262 was down-regulated in cervical cancer tissues and cells. We found that CRNDE sponged miR-4262 and ZEB1 was a target of miR-4262. In addition, miR-4262 inhibitor abolished CRNDE silencing-induced repression on cell proliferation, EMT, migration, invasion and promotion on cell apoptosis. Furthermore, ZEB1 rescued the effects of miR-4262 overexpression or CRNDE deletion on cervical cancer progression. Our data showed that CRNDE targeted miR-4262 to regulate ZEB1 expression in cervical cancer cells. Besides, CRNDE expedited cervical cancer progression through wnt/β-catenin pathway via sponging miR-4262 and altering ZEB1 expression.Conclusion: Our findings demonstrated that CRNDE facilitated the progression of cervical cancer through activation of wnt/β-catenin pathway by regulating miR-4262/ZEB1 axis, representing a prospective targeted therapy for cervical cancer.Keywords: CRNDE, miR-4262, ZEB1, wnt/β-catenin pathway, cervical cancer |
| Databáze: | OpenAIRE |
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