Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization
Autor: | Suk-Yul Jung, A-Young Park, Sutherland K. Maciver, Ho-Joon Shin, Naveed Ahmed Khan, A-Young Kang |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.drug_class Immunology Blotting Western Protozoan Proteins Antibodies Protozoan Acanthamoeba Antigens Protozoan Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody Mannose-Binding Lectin Microbiology 03 medical and health sciences Mice Affinity chromatography parasitic diseases medicine Animals Mannan-binding lectin Mice Inbred BALB C Hybridomas Immune Sera Antibodies Monoclonal Acanthamoeba infection General Medicine 030108 mycology & parasitology medicine.disease biology.organism_classification Immunohistochemistry eye diseases Immunoglobulin Isotypes Infectious Diseases Acanthamoeba keratitis Acanthamoeba Keratitis biology.protein Acanthamoeba castellanii Parasitology Electrophoresis Polyacrylamide Gel Antibody |
Zdroj: | Kang, A-Y, Park, A-Y, Shin, H-J, Khan, N A, Maciver, S & Jung, S-Y 2018, ' Production of a monoclonal antibody against a mannose-binding protein of Acanthamoeba culbertsoni and its localization ', Experimental Parasitology, vol. 192 . https://doi.org/10.1016/j.exppara.2018.07.009 |
ISSN: | 1090-2449 |
Popis: | Amoebae from the genus Acanthamoeba are facultative pathogens of humans and other animals. In humans they most frequently infect the eye causing a sight threatening infection known as Acanthamoeba keratitis (AK), and also cause an often fatal encephalitis (GAE). A mannose-binding protein (MBP) has been identified as being important for Acanthamoeba infection especially in AK. This lectin has previously been characterized from Acanthamoeba castellanii as consisting of multiple 130 kDa subunits. MBP expression correlates with pathogenic potential and is expressed in a number of Acanthamoeba species. Here we report the purification of a similar lectin from Acanthamoeba culbertsoni and the production of a monoclonal antibody to it. The A. culbertsoni MBP was isolated by affinity chromatography using α-D-mannose agarose and has an apparent molecular weight of 83 kDa. The monoclonal antibody is an IgM that is useful in both western blots and immunofluorescence. We expect that this antibody will be useful in the study of the pathology of A. culbertsoni and in its identification in clinical samples. |
Databáze: | OpenAIRE |
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