Characterization of glycans in the developmental stages of Myxobolus cerebralis (Myxozoa), the causative agent of whirling disease
| Autor: | M Stippl, H Kaltner, Mansour El-Matbouli, Martin Knaus |
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| Rok vydání: | 2007 |
| Předmět: |
Glycan
Veterinary (miscellaneous) Spores Protozoan Aquatic Science Sambucus nigra Fish Diseases Agglutinin Antigen Polysaccharides Cell Adhesion Animals Oligochaeta Protozoan Infections Animal Myxobolus cerebralis biology food and beverages Lectin Eukaryota biology.organism_classification Ulex europaeus Molecular biology Complement system Cartilage Oncorhynchus mykiss Immunology biology.protein Plant Lectins |
| Zdroj: | Journal of fish diseases. 30(11) |
| ISSN: | 0140-7775 |
| Popis: | Glycans and sugar-binding molecules (lectins) form an interactive recognition system, which may enable parasitic organisms to adhere to host cells and migrate into target tissues. The aim of the present study was to analyse surface-associated glycans in the developmental stages of Myxobolus cerebralis (Hofer), the causative agent of whirling disease. A panel of biotin-labelled plant lectins was used to detect a broad spectrum of glycan motifs with high specificity. Binding sites were detected histochemi- cally in the tissue sections of infected rainbow trout, Oncorhynchus mykiss (Walbaum), and infected Tubifex tubifex (Muller), and were characterized by light, fluorescence and transmission electron microscopy. With mannose-specific lectins (Lens culinaris agglutinin, Pisum sativum agglutinin, Canavalia ensiformis agglutinin (LCA, PSA, CanA)) mannose-containing glycans were detected in all the developmental stages and host tissues. No binding sites for galactose-specific lectins were present in M. cerebralis spores but reactivity with host tissues occurred. Diversity in glycans was detected by N-acetyl-D-galactosamine-specific lectins in sporo- plasm cells of M. cerebralis and triactinomyxon spores. In the group of lectins with monosaccharide- specificity for N-acetyl-D-glucosamine (GlcNAc), the reactivity of Datura stramonium agglutinin (DSA), Lycopersicon esculentum agglutinin (LEA) and Solanum tuberosum agglutinin (STA) was restricted to polar capsules whereas Griffonia sim- plicifolia agglutinin II (GSA II) also bound to spo- roplasm cells of stages in the fish host but not in those present in infected T. tubifex. Moreover, Triticum vulgaris (wheat germ) agglutinin (WGA) and succinylated WGA indicated the presence of N-acetyl-D-glucosamine polymers in polar capsules. No specificity for spores was observed concerning bisected N-glycans and no reactivity in parasitic stages was observed with the fucose-binding lectin Ulex europaeus agglutinin (UEA) I, Sambucus nigra agglutinin (SNA) (specific for a2,6-sialylated gly- cans) and Maackia amurensis agglutinin (MAAI) (specific for a2,3-sialylated glycans). Arachis hypo- gaea (peanut) agglutinin (PNA), Erythrina cristagalli agglutinin (ECA), GSA I, Sophora japonica aggluti- nin (SJA), Dolichos biflorus agglutinin (DBA) and GSA II detected reactive sites solely confined to the developmental stages of M. cerebralis and were not reactive in the fish host. These parasite-specific gly- cans may play a role in the adhesion process of the parasite to fish epidermis prior to infection, but may provide protection to the host by activating the complement system, or stimulating an adaptive immune response as putative antigens. |
| Databáze: | OpenAIRE |
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