Diversity of coelomycetous fungi in human infections: A 10-y experience of two European reference centres

Autor: Garcia-Hermoso, Dea, Valenzuela-Lopez, Nicomedes, Rivero-Menendez, Olga, Alastruey-Izquierdo, Ana, Guarro, Josep, Cano-Lira, José, Stchigel, Alberto, French Mycoses Study Group, The
Přispěvatelé: Institut Pasteur [Paris], Centre National de Référence Mycologie et Antifongiques-Mycologie Moléculaire (CNRMA), Mycologie moléculaire - Molecular Mycology, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Universitat Rovira i Virgili, Universidad de Antofagasta, Instituto de Salud Carlos III [Madrid] (ISC), This work was supported by the Spanish Ministerio de Economía y Competitividad, grant CGL2017-88094-P.Olga Rivero-Menendez holds a fellowship from the Fondo de Investigaciones Sanitarias (grant FI14CIII/00025). Ana Alastruey-Izquierdo is supported by a research project from the Fondo de Investigación Sanitaria (FIS) (project PI16CIII/00035)., We thank Cécile Gautier (National Reference Center for invasive Mycoses and Antifungals [NRCMA]) for technical assistance., Members of the French Mycoses Study Group who contributed their clinical isolates to this study are as follows: Annecy (S. Bland), Bichat (C. Chochillon), Boulogne-Billancourt (N.Ait-Ammar), Caen (C. Duhamel), Clermont Ferrand (P.Poirier), Cochin (A. Paugam), Corbeil-Essones (D. Kubab), Guadeloupe (M. Nicolas), La Réunion (S. Picot), Lyon (A.L. Bienvenu), Martinique (N. Desbois), Nantes (F. Morio), Necker (M.E. Bougnoux), Nouvelle Calédonie (R. Goursaud), Pitié (A. Fekkar), Poitiers (C. Kauffmann), Quinze-Vingts (L. Merabet), Rouen (L. Favennec), Saint Etienne (H. Raberin), Saint-Louis (A. Alanio), Toulouse (S. Cassaing)., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0106 biological sciences
MESH: Sequence Analysis
DNA
Antifungal Agents
Phoma
Coelomycetes
01 natural sciences
chemistry.chemical_compound
RNA
Ribosomal
28S
Cluster Analysis
Pleosporales
DNA
Fungal
MESH: Phylogeny
Phylogeny
Diaporthales
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
0303 health sciences
MESH: Microbial Sensitivity Tests
MESH: DNA
Ribosomal
biology
Medicopsis
Antifungal testing
3. Good health
MESH: RNA
Ribosomal
28S
Infectious Diseases
France
MESH: Fungi
Context (language use)
Microbial Sensitivity Tests
DNA
Ribosomal
Microbiology
Mycosis
03 medical and health sciences
MESH: Mycoses
Genetics
Colletotrichum
MESH: Spain
Humans
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
MESH: Humans
Fungi
Sequence Analysis
DNA
biology.organism_classification
MESH: Antifungal Agents
MESH: Cluster Analysis
MESH: DNA
Fungal
MESH: France
Mycoses
chemistry
Spain
Didymellaceae
Caspofungin
010606 plant biology & botany
Zdroj: Fungal Biology
Fungal Biology, Elsevier, 2019, 123 (4), pp.341-349. ⟨10.1016/j.funbio.2019.02.001⟩
Fungal Biology, 2019, 123 (4), pp.341-349. ⟨10.1016/j.funbio.2019.02.001⟩
ISSN: 1878-6146
Popis: International audience; The coelomycetous fungi are difficult to properly identify from their phenotypic characterization and their role as etiologic agents of human infections is not clear. We studied the species distribution of these fungi among clinical isolates that had been collected and stored over a ten-year period in two European reference laboratories (France and Spain). We identified phenotypically and molecularly 97 isolates by sequencing the D1-D2 fragment of the 28S nrRNA (LSU) gene and we provided the in vitro antifungal susceptibility pattern of seven antifungals against 46 isolates. Species of the orders Pleosporales and Glomerellales were present in both collections, and Botryosphaeriales and Diaporthales only in the French one. The most prevalent species were Medicopsis romeroi, Neocucurbitaria keratinophila, Neocucurbitaria unguis-hominis and Paraconiothyrium cyclothyrioides, which had been recovered primarily from superficial tissues. The Didymellaceae was the most common family represented, with 27 isolates distributed into five genera. Most of the isolates tested were susceptible to antifungals, and only the geometric mean (GM) and minimal inhibitory concentration (MIC) values of itraconazole and caspofungin had higher values. This study provides a good picture of the great diversity of coelomycetous fungi in the European clinical context, and the basis for future studies on this interesting but neglected group of fungi.
Databáze: OpenAIRE
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