Anti-Inflammatory Local Effect of Hydroxytyrosol Combined with Pectin-Alginate and Olive Oil on Trinitrobenzene Sulfonic Acid-Induced Colitis in Wistar Rats
| Autor: | A Voltes, F. de la Portilla, G Rodríguez-Gutiérrez, C Olano, J Fernández-Bolaños, A Bermúdez, Maria L. Reyes |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission |
| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
Pectin Alginates medicine.drug_class Anti-Inflammatory Agents Enema Pharmacology Sulfonic acid Inflammatory bowel disease Antioxidants Anti-inflammatory 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food medicine Animals Humans Hydroxytyrosol Intestinal Mucosa Rats Wistar Colitis Olive Oil chemistry.chemical_classification business.industry Pectin/alginate Rectum Inflammatory Bowel Diseases Phenylethyl Alcohol medicine.disease Rats Disease Models Animal Trinitrobenzenesulfonic Acid chemistry 030220 oncology & carcinogenesis Feasibility Studies Pectins Drug Therapy Combination Female 030211 gastroenterology & hepatology Surgery business Olive oil |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1521-0553 0894-1939 |
| DOI: | 10.1080/08941939.2018.1469697 |
| Popis: | Purpose: Evaluate the efficacy of hydroxytyrosol in the local treatment of inflammatory colitis. Currently, the existing treatments for inflammatory bowel diseases does not cure the disease and it is associated with high rates of side effects and complications. Hydroxytyrosol is a phenyl-ethyl-alcohol derived from the hydrolysis of oleuropein and present in olive oil, previous studies have demonstrated the anti-inflammatory effect of dietary hydroxytyrosol supplement, with no toxicity. Materials & Methods: Colitis has been induced by using Trinitrobenzene Sulfonic Acid at 40 rats. They were divided into four groups randomly: 10 rats without treatment; 10 rats with pectin/alginate mixture; 10 rats treated with pectin/alginate + olive oil; 10 rats treated with pectin/alginate + olive oil + hydroxytyrosol. Animals were sacrificed 10 days after induction of trinitrobenzene sulfonic acid, receiving 5 days of continuous treatment. Samples of the rectal area were studied and observed under a microscope to determine the damage by Hunter scoring modified, assessing inflammatory infiltration, number of intestinal walls involved, damage to the mucosal architecture, and edema. Results: When the rectum was analyzed in a global way, nonsignificant differences were observed; however, when performing an individualized analysis, statistically significant differences in the inflammatory infiltrate are present in the samples, which were evaluated using the ANOVA and Student-T statistics. Conclusions: Local treatment with the natural antioxidant hydroxytyrosol combined with pectin/alginate and olive oil of inflammatory bowel disease has been shown to be effective against inflammatory infiltration of TNBS-induced colitis. This research was supported by the Spanish Min-istry of Economy, Industry and Competitivenessand cofunded by a European Social Fund (ESF)(Ramon y Cajal Programme RyC 2012–10456; projectAGL2013–48291-R and project AGL2016-79088-R) andA. Bermúdez-Oria received funding from the SpanishFPI program (MEIC) (BES-2014-068508) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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