A fission yeast strain expressing human CDC25A phosphatase: a tool for selectivity studies of pharmacological inhibitors of CDC25

Autor: Marie Odile Galera-Contour, Matthieu Lemaire, Gregoire Prevost, Bernard Ducommun, Béatrix Bugler, Odile Mondesert, Marie-Christine Brezak
Přispěvatelé: Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération (LBCMCP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre de Biologie Intégrative (CBI), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Henri Beaufour (IPSEN), IPSEN-BEAUFOUR
Rok vydání: 2003
Předmět:
Time Factors
Cell Cycle Proteins
MESH: Protein Isoforms
MESH: Dose-Response Relationship
Drug
MESH: Genotype
0302 clinical medicine
MESH: cdc25 Phosphatases
Hydroxyurea
Protein Isoforms
MESH: Models
Genetic
Enzyme Inhibitors
MESH: Inhibitory Concentration 50
0303 health sciences
biology
MESH: DNA
Temperature
General Medicine
MESH: ras-GRF1
MESH: Hydroxyurea
MESH: Temperature
3. Good health
MESH: Schizosaccharomyces
Biochemistry
MESH: Enzyme Inhibitors
030220 oncology & carcinogenesis
MESH: Phosphoric Monoester Hydrolases
MESH: Fungal Proteins
biological phenomena
cell phenomena
and immunity
MESH: Infrared Rays
Plasmids
CDC25A
Genotype
Cdc25
Infrared Rays
Ultraviolet Rays
Phosphatase
MESH: Thiazoles
Mitosis
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Fungal Proteins
03 medical and health sciences
Inhibitory Concentration 50
MESH: Cell Cycle Proteins
MESH: Plasmids
MESH: Cell Proliferation
MESH: Dose-Response Relationship
Radiation
Schizosaccharomyces
Genetics
Humans
cdc25 Phosphatases
Benzothiazoles
MESH: Benzothiazoles
030304 developmental biology
Cell Proliferation
MESH: DNA Damage
MESH: Humans
Dose-Response Relationship
Drug
Models
Genetic
ras-GRF1
MESH: Time Factors
Dose-Response Relationship
Radiation
DNA
G2-M DNA damage checkpoint
MESH: Mitosis
biology.organism_classification
Yeast
Phosphoric Monoester Hydrolases
enzymes and coenzymes (carbohydrates)
Thiazoles
Schizosaccharomyces pombe
biology.protein
MESH: Ultraviolet Rays
Homologous recombination
DNA Damage
Zdroj: Current Genetics
Current Genetics, Springer Verlag, 2004, 45 (5), pp.283-8. ⟨10.1007/s00294-003-0483-3⟩
ISSN: 0172-8083
1432-0983
DOI: 10.1007/s00294-003-0483-3⟩
Popis: Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous Cdc25 mitotic inducer for vegetative growth and (b). that CDC25A is able to partially restore a functional checkpoint in response to both ionising and UV irradiation, but not a DNA replication checkpoint. We then describe a simple assay in which we demonstrate that growth of the humanised CDC25A strain is strongly repressed in a CDC25-dependent manner by BN2003, a potent chemical inhibitor of CDC25 belonging to the benzothiazoledione family. The ease of manipulation of fission yeast humanised CDC25 cells and the simplicity of the above assay offer a powerful tool with which to investigate the specificity of pharmacological inhibitors of CDC25.
Databáze: OpenAIRE
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