The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae
| Autor: | Zhixin Liu, Heiko Wagner, Katja Stehfest, Uwe Langner, Christian Wilhelm |
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| Rok vydání: | 2010 |
| Předmět: |
Absorption (pharmacology)
Calibration curve General Engineering Analytical chemistry food and beverages General Physics and Astronomy General Chemistry Biology General Biochemistry Genetics and Molecular Biology Metabolic engineering symbols.namesake Fourier transform Bacterial Proteins Spectroscopy Fourier Transform Infrared Microalgae symbols Bioreactor General Materials Science Fourier transform infrared spectroscopy Spectroscopy Chlamydomonas reinhardtii Macromolecule |
| Zdroj: | Journal of Biophotonics. 3:557-566 |
| ISSN: | 1864-063X |
| DOI: | 10.1002/jbio.201000019 |
| Popis: | A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate and lipid contents of microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by a partial least square algorithm, using the spectra of the reference substances to determine their relative contribution to the overall cell spectrum. From this specific absorption, absolute macromolecular cell composition [pg cell(-1)] can be calculated using calibration curves, which have been validated by independent biochemical methods. The future potential of this method for photosynthesis research is shown by its application to follow time-resolved changes in the cellular composition of microalgae during an illumination period of several hours. We show how the macromolecular composition can be investigated by FTIR spectroscopy methods. This can substantially increase the efficiency of screening processes like bioreactor monitoring and may be beneficial in metabolic engineering of algal cells. |
| Databáze: | OpenAIRE |
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