The identification of elevated urinary kisspeptin-immunoreactivity during pregnancy
| Autor: | Mansimran Cheema, G. M.K. Nijher, Channa N. Jayasena, Shakunthala Narayanaswamy, Ali Abbara, Waljit S. Dhillo, Alexander N Comninos, Stephen R. Bloom, M. A. Ghatei, Zainab Malik |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Saliva Pregnancy Trimester Third Urinary system Clinical Biochemistry Radioimmunoassay Pilot Projects Urine Peptide hormone Kisspeptin Pregnancy Internal medicine Placenta medicine Humans Kisspeptins business.industry Gestational age General Medicine medicine.disease Endocrinology medicine.anatomical_structure Female business Biomarkers hormones hormone substitutes and hormone antagonists |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 52:395-398 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1177/0004563214551612 |
| Popis: | Background Kisspeptin is an arginine-phenylalanine amide peptide hormone critical for reproductive function. Kisspeptin is also abundantly expressed in the placenta, where it has an important physiological role in regulating placental invasion. Accordingly, plasma kisspeptin concentrations rise dramatically during normal pregnancy. However, lower plasma concentrations of kisspeptin are associated with obstetric complications such as pre-eclampsia. It is not currently known whether kisspeptin-immunoreactivity (IR) can be detected in bodily fluids not requiring invasive collection such as saliva or urine. Aim To determine the clinical utility of urinary and salivary kisspeptin measurement in healthy pregnant women. Methods Forty-nine healthy third trimester pregnant women (gestational age 34 ± 0.6 w) from a single maternity unit and 50 healthy non-pregnant women were recruited. Urine, saliva and blood were simultaneously collected from all volunteers. Kisspeptin concentrations were determined by in-house manual radioimmunoassay. Results Mean concentrations of plasma kisspeptin-IR were over 200-fold greater in third trimester pregnant women compared with non-pregnant women (13,783 ± 864 pmol/L, pregnant; 65 ± 13 pmol/L, non-pregnant; p Conclusion We demonstrate for the first time that kisspeptin-IR is elevated in urine during pregnancy. Urinary measurement of kisspeptin-IR may, therefore, offer a non-invasive and simple method of screening for pregnancy and obstetric complications. |
| Databáze: | OpenAIRE |
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