The Effects of a Single Freeze-Thaw Cycle on Concentrations of Nutritional, Noncommunicable Disease, and Inflammatory Biomarkers in Serum Samples
| Autor: | Krishnapillai Madhavan Nair, Raghu Pullakhandam, Ransi Ann Abraham, Sucheta Banerjee Kurundkar, Arvind Pandey, Sowmya Ramesh, Nizamuddin Khan, Garima Rana, Arjan de Wagt, Ajay Khera, Akash Porwal, Robert Johnston, Avina Sarna, Praween K. Agrawal, Sila Deb, Geeta Trilok Kumar, H P S Sachdev, Umesh Kapil, Lakshmy Ramakrishnan, Rajib Acharya |
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| Rok vydání: | 2021 |
| Předmět: |
030213 general clinical medicine
business.industry analysis Physiology nutrition and noncommunicable diseases biomarkers 030204 cardiovascular system & hematology stability Serum samples Inflammatory biomarkers storage 03 medical and health sciences 0302 clinical medicine Paired samples Noncommunicable disease freeze-thaw Medicine Original Article business bioanalytes serum sample |
| Zdroj: | Journal of Laboratory Physicians Journal of Laboratory Physicians, Vol 13, Iss 01, Pp 006-013 (2021) |
| ISSN: | 0974-2727 |
| Popis: | Background The stability of biological samples is vital for reliable measurements of biomarkers in large-scale survey settings, which may be affected by freeze-thaw procedures. We examined the effect of a single freeze-thaw cycle on 13 nutritional, noncommunicable diseases (NCD), and inflammatory bioanalytes in serum samples. Method Blood samples were collected from 70 subjects centrifuged after 30 minutes and aliquoted immediately. After a baseline analysis of the analytes, the samples were stored at − 70°C for 1 month and reanalyzed for all the parameters. Mean percentage differences between baseline (fresh blood) and freeze-thaw concentrations were calculated using paired sample t-tests and evaluated according to total allowable error (TEa) limits (desirable bias). Results Freeze-thaw concentrations differed significantly (p < 0.05) from baseline concentrations for soluble transferrin receptor (sTfR) (− 5.49%), vitamin D (− 12.51%), vitamin B12 (− 3.74%), plasma glucose (1.93%), C-reactive protein (CRP) (3.45%), high-density lipoprotein (HDL) (7.98%), and cholesterol (9.76%), but they were within respective TEa limits. Low-density lipoprotein (LDL) (− 0.67%), creatinine (0.94%), albumin (0.87%), total protein (1.00%), ferritin (− 0.58%), and triglycerides (TAG) (2.82%) concentrations remained stable following the freeze-thaw cycle. In conclusion, single freeze-thaw cycle of the biomarkers in serum/plasma samples after storage at − 70°C for 1 month had minimal effect on stability of the studied analytes, and the changes in concentration were within acceptable limit for all analytes. |
| Databáze: | OpenAIRE |
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