Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3-beta-HSD and ARO but lower 5-alpha-R-1 in male rats

Autor: Maria Florencia Andreoli, Jorge G. Ramos, Cora Stoker, Gisela Paola Lazzarino, Enrique H. Luque, María Florencia Rossetti
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
Cholestenone 5 alpha-Reductase
Endocrinology
Diabetes and Metabolism
Gene Expression
Estrogen receptor
Eating
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Neurotransmitter Agents
Nutrition and Dietetics
Steroidogenic acute regulatory protein
food and beverages
purl.org/becyt/ford/3.1 [https]
Medicina Básica
Hypothalamus
purl.org/becyt/ford/3 [https]
Signal Transduction
endocrine system
medicine.medical_specialty
3-Hydroxysteroid Dehydrogenases
CIENCIAS MÉDICAS Y DE LA SALUD
Neuroactive steroid
Neurociencias
Alpha (ethology)
Phytoestrogens
Biology
Diet
High-Fat
03 medical and health sciences
Aromatase
Internal medicine
medicine
Animals
Obesity
Rats
Wistar
Steroidogenic Enzymes
Appetite Regulation
urogenital system
Cholesterol side-chain cleavage enzyme
Allopregnanolone
Estrogen Receptor alpha
Diet
030104 developmental biology
chemistry
Energy Intake
030217 neurology & neurosurgery
Erα
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 0271-5317
DOI: 10.1016/j.nutres.2016.05.003
Popis: Removing dietary phytoestrogens causes obesity and diabetes in adult male rats. Based on the facts that hypothalamic food intake control is disrupted in phytoestrogen-deprived animals and that several steroids affect food intake, we hypothesized that phytoestrogen withdrawal alters the expression of hypothalamic steroidogenic enzymes. Male Wistar rats fed with a high-phytoestrogen diet from conception to adulthood were subjected to phytoestrogen withdrawal by feeding them a low-phytoestrogen diet or a high-phytoestrogen, high-fat diet. Withdrawal of dietary phytoestrogens increased 3β-hydroxysteroid dehydrogenase and P450 aromatase gene expression and decreased those of 5α-reductase-1. This is a direct effect of the lack of dietary phytoestrogens and not a consequence of obesity, as it was not observed in high-fat–fed rats. Phytoestrogen withdrawal and high-fat diet intake reduced hypothalamic expression of estrogen receptor (ER)α correlated with low levels of ERα-O, ERα-OS, and ERα-OT transcripts. Variations in gene expression of steroidogenic enzymes may affect the content of neurosteroids. As neurosteroids are related to food intake control, the changes observed may be a novel mechanism in the regulation of energy balance in obese phytoestrogen-deprived animals. In rats, steroidogenesis and ER signaling appear to be altered by phytoestrogen withdrawal in the rat. The ubiquity of phytoestrogens in the diet and changing intakes or withdrawal suggest that aspects of human health could be affected based on the rat and warrant further research. Fil: Andreoli, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Stoker, Cora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Lazzarino, Gisela Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Databáze: OpenAIRE
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