Antigen-responsive fluorescent antibody probes generated by selective N-terminal modification of IgGs
| Autor: | Fukunaga, Keisuke, Watanabe, Takayoshi, Novitasari, Dian, Ohashi, Hiroyuki, Abe, Ryoji, Hohsaka, Takahiro |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
biology Chemistry IgG.monoclonal Metals and Alloys General Chemistry Alkylation 010402 general chemistry 01 natural sciences Small molecule Fluorescence Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials 03 medical and health sciences Fluorescent labelling 030104 developmental biology Biochemistry Antigen Materials Chemistry Ceramics and Composites biology.protein Antibody |
| Zdroj: | Chemical Communications. 54:12734-12737 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c8cc07827k |
| Popis: | Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to detect antigens in a rapid and quantitative manner. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|