Zebrafish genomic library in yeast artificial chromosomes
| Autor: | Maryann Haldi, Mark C. Fishman, Uma Akella, Kim Kaphingst, Tao P. Zhong, Eric S. Lander |
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| Rok vydání: | 1998 |
| Předmět: |
Genetics
Yeast artificial chromosome congenital hereditary and neonatal diseases and abnormalities Genomic Library biology fungi hemic and immune systems chemical and pharmacologic phenomena DNA Molecular cloning biology.organism_classification Insert (molecular biology) law.invention genomic DNA law hemic and lymphatic diseases Animals Genomic library Cloning Molecular Zebrafish Chromosomes Artificial Yeast Polymerase chain reaction Genomic organization |
| Zdroj: | Genomics. 48(1) |
| ISSN: | 0888-7543 |
| Popis: | We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones. |
| Databáze: | OpenAIRE |
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