Spheroids as a Type of Three-Dimensional Cell Cultures—Examples of Methods of Preparation and the Most Important Application
| Autor: | Katarzyna Milowska, Piotr Komorowski, Kamila Białkowska, Maria Bryszewska |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Cell Cell Culture Techniques spheroids Review Catalysis In vitro model Inorganic Chemistry lcsh:Chemistry 3D cell culture Spheroids Cellular medicine Humans Physical and Theoretical Chemistry Molecular Biology lcsh:QH301-705.5 drug testing Spectroscopy Cells Cultured Chemistry Organic Chemistry Spheroid Hydrogels General Medicine Computer Science Applications Cell biology Extracellular Matrix medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 Cell culture Self-healing hydrogels |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 21, Iss 6225, p 6225 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Cell cultures are very important for testing materials and drugs, and in the examination of cell biology and special cell mechanisms. The most popular models of cell culture are two-dimensional (2D) as monolayers, but this does not mimic the natural cell environment. Cells are mostly deprived of cell–cell and cell–extracellular matrix interactions. A much better in vitro model is three-dimensional (3D) culture. Because many cell lines have the ability to self-assemble, one 3D culturing method is to produce spheroids. There are several systems for culturing cells in spheroids, e.g., hanging drop, scaffolds and hydrogels, and these cultures have their applications in drug and nanoparticles testing, and disease modeling. In this paper we would like to present methods of preparation of spheroids in general and emphasize the most important applications. |
| Databáze: | OpenAIRE |
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