Discovery of a novel insecticidal protein from Chromobacterium piscinae , with activity against Western Corn Rootworm, Diabrotica virgifera virgifera
| Autor: | Maria Stauffer, Brian Mcnulty, Rong Guo, Daniel J. Tomso, Brian Vande Berg, Nalini Desai, Kimberly S. Sampson, Deepa Balasubramanian, Jelena Zaitseva |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Sequence analysis Biology medicine.disease_cause Polymerase Chain Reaction Zea mays 01 natural sciences Mass Spectrometry Microbiology 03 medical and health sciences Botany medicine Animals Bioassay Pest Control Biological Gene Ecology Evolution Behavior and Systematics Larva Genetically modified maize Toxin Chromobacterium Biological activity Plants Genetically Modified biology.organism_classification Coleoptera Endotoxins 010602 entomology 030104 developmental biology Western corn rootworm |
| Zdroj: | Journal of Invertebrate Pathology. 142:34-43 |
| ISSN: | 0022-2011 |
| Popis: | Western corn rootworm (WCR), Diabrotica virgifera virgifera, is one of the most significant pests of corn in the United States. Although transgenic solutions exist, increasing resistance concerns make the discovery of novel solutions essential. In order to find a novel protein with high activity and a new mode of action, a large microbial collection was surveyed for toxicity to WCR using in vitro bioassays. Cultures of strain ATX2024, identified as Chromobacterium piscinae, had very high activity against WCR larvae. The biological activity from the strain was purified using chromatographic techniques and fractions were tested against WCR larvae. Proteins in the final active fraction were identified by mass spectrometry and N-terminal sequencing and matched to the genome of ATX2024. A novel 58.9kDa protein, identified by this approach, was expressed in a recombinant expression system and found to have specific activity against WCR. Transgenic corn events containing this gene showed good protection against root damage by WCR, with average scores ranging between 0.01 and 0.04 on the Iowa State node injury scale. Sequence analysis did not reveal homology to any known insecticidal toxin, suggesting that this protein may act in a novel way to control WCR. The new WCR active protein is named GNIP1Aa, for Gram Negative Insecticidal Protein. |
| Databáze: | OpenAIRE |
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