Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi
| Autor: | Jing Fen Jia, Da Luo, Jiang Bo Wang, Yu Mei Wang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Acetosyringone
food.ingredient biology Agrobacterium fungi food and beverages Fabaceae Opine Cell Biology Plants Genetically Modified biology.organism_classification Plant Roots Hypocotyl Alhagi chemistry.chemical_compound Transformation (genetics) Transformation Genetic food chemistry Callus Botany Regeneration Molecular Biology Cotyledon Rhizobium |
| Zdroj: | Cell Research. 11:279-284 |
| ISSN: | 1748-7838 1001-0602 |
| DOI: | 10.1038/sj.cr.7290097 |
| Popis: | The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Regenerated plants were achieved from sterile calli derived from hairy roots, which occurred at or near the infection sites. The regenerated plants from hairy root were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T-DNA had been integrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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