RIR1 represses plant immunity by interacting with mitochondrial complex I subunit in rice
| Autor: | Yantao Jia, Rongxiang Fang, Ping Guo, Liyang Song, Lin An, Hongyan Guo, Siyuan Zhang, Chuanmiao Xie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Xanthomonas
Protein subunit Mutant Soil Science Repressor Plant Science Biology Type three secretion system OsmiR159a.1 chemistry.chemical_compound Xanthomonas oryzae Gene Expression Regulation Plant Plant Immunity Molecular Biology Gene Plant Diseases Plant Proteins Electron Transport Complex I Jasmonic acid food and beverages ROS Oryza Original Articles biology.organism_classification Cell biology Complementation chemistry Original Article LRR_RLK Agronomy and Crop Science mitochondrial complex I |
| Zdroj: | Molecular Plant Pathology |
| ISSN: | 1364-3703 1464-6722 |
| Popis: | We previously observed decreased expression of rice OsmiR159a.1 on infection with the bacterial blight‐causing pathogen Xanthomonas oryzae pv. oryzae (Xoo), and identified the OsLRR_RLK (leucine‐rich repeat_ receptor like kinase) gene as an authentic target of OsmiR159a.1. Here, we found that a Tos17 insertion mutant of LRR_RLK displayed increasing temporal resistance to Xoo, whereas the LRR_RLK overexpression lines were susceptible to the pathogen early on in the infection, indicating that LRR_RLK encodes a repressor of rice resistance to Xoo infection, and it was renamed as RIR1 (Rice Immunity Repressor 1). RIR1 overexpression plants were more susceptible to Xoo at late growth stage, suggesting that RIR1 mRNA levels are negatively correlated with the resistance of rice against Xoo. We discovered that OsmiR159a.1 repression in Xoo‐infected plants was largely dependent on the pathogen's type III secretion system. Co‐immunoprecipitation, bimolecular fluoresence complementation, and pull‐down assays indicated that RIR1 interacted with the NADH‐ubiquinone oxidoreductase (NUO) 51‐kDa subunit of the mitochondrial complex I through its kinase domain. Notably, impairment of RIR1 or overexpression of NUO resulted in reactive oxygen species accumulation and enhanced expression of pathogen‐resistance genes, including jasmonic acid pathway genes. We propose that pathogens may inhibit OsmiR159 to interfere with the RIR1–NUO interaction, and subsequently depression of rice immune signalling pathways. The resistance genes manipulated by Xoo can be a probe to explore the regulatory network during host–pathogen interactions. Xanthomonas oryzae may use type III effectors to inhibit OsmiR159 and release its target rice gene RIR1 expression, then promote RIR1–NUO interaction that leads to depression of rice immune signalling pathways. |
| Databáze: | OpenAIRE |
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