Detection of Pneumocystis in the nasal swabs of immune-suppressed rats by use of PCR and microscopy
| Autor: | Sabire Karaçali, Yüksel Gürüz, Aysu Değirmenci, Mert Döşkaya, Ceylan Polat, Ahmet Üner, Hüseyin Can, Ayşe Caner |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Pathology medicine.medical_specialty diagnosis Genes Fungal Nose Biology Pneumocystis carinii Pneumocystis pneumonia Polymerase Chain Reaction Nasopharyngeal aspirate medicine Animals Dexamethasone Immunosuppression Therapy Microscopy nasal swab Pneumonia Pneumocystis Fungal genetics General Medicine respiratory system colonization medicine.disease Rats respiratory tract diseases PCR medicine.anatomical_structure Nasal Swab Animal Studies Pneumonia (non-human) medicine.drug |
| Zdroj: | Medical Science Monitor Basic Research |
| ISSN: | 2325-4416 |
| Popis: | Background Detection of Pneumocystis jiroveci colonization in lungs or oral samples due to high sensitivity of PCR methods results in undue treatment of patients without any symptoms of Pneumocystis pneumonia. The aim of the present study is to demonstrate Pneumocystis carinii in rats, immune suppressed by oral and subcutaneous administration of dexamethasone. Material/Methods Blood, oral, nasal and eye swabs were collected prior to immune suppression and 2, 6, 12 weeks after administration of dexamethasone. Also, samples were collected from lung, heart, liver, kidney, diaphragm, brain, spleen, tongue, muscle, eye, intestine, and feces. Cysts and trophozoites were investigated in stained slides and MSG gene was detected by PCR. Results The results showed that weight loss is significantly higher in rats administered oral dexamethasone (P |
| Databáze: | OpenAIRE |
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