Molecular cloning and characterization of Cpn60 in the free-living nematode Plectus acuminatus
| Autor: | Stephen R. Stürzenbaum, M S J Arts, J E Kammenga |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
chaperonin
Nematoda Short Communication Molecular Sequence Data Zoology Environmental pollution Molecular cloning heat-shock Biochemistry chemistry.chemical_compound Chlorides Heat shock protein expression stress-proteins regions Animals Amino Acid Sequence gene Peptide sequence Gene Laboratorium voor Nematologie Genetics Messenger RNA biology Temperature autoimmune Cell Biology Chaperonin 60 Sequence Analysis DNA biology.organism_classification PE&RC shock-protein hsp60 Nematode chemistry Zinc Compounds identification Laboratory of Nematology atherosclerosis DNA |
| Zdroj: | Cell Stress and Chaperones, 10(2), 79-85 Cell Stress and Chaperones 10 (2005) 2 |
| ISSN: | 1355-8145 |
| Popis: | Heat shock proteins (Hsps) have provoked interest not only because of their involvement in human diseases but also for their potential as biomarkers of environmental pollution. Whereas the former interest is covered by numerous reports, the latter is an exciting new field of research. We report the isolation of the full-length cpn60 messenger ribonucleic acid (mRNA) and partial genomic deoxyribonucleic acid from the free-living, environmental sentinel nematode Plectus acuminatus, a species used in classical ecotoxicity tests. Although the primary sequence displays high identity scores to other nematodes and human Cpn60 (75% and 70%, respectively), the intron-exon structure differs markedly. Furthermore, although mRNA levels remained constant after exposure to ZnCl2 (0-330 microM) under laboratory conditions, protein levels increased significantly in a dose-dependent manner. In conclusion, this first account of molecular genetic similarities and differences of Cpn60 in a neglected nematode taxon provides a valuable insight into its potential uses in gene-based ecotoxicological risk assessment exercises. |
| Databáze: | OpenAIRE |
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