Cytogenetic evidence supports Avena insularis being closely related to hexaploid oats
| Autor: | E. Ferrer, J. M. González, A. Fominaya, Yolanda Loarce |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
food.ingredient
Avena DNA Plant Science Biology Hexaploidy Genome Homology (biology) Chromosomes Plant Chromosomes Polyploidy Cytogenetics food Cell Signaling Fish Genomics Genetics Repeated sequence Phylogeny Multidisciplinary Chromosome Biology Autosomes fungi Chromosome Biology and Life Sciences food and beverages Karyotype Genomics Cell Biology Ribosomal RNA Chromosome Pairs Chromosomal Aberrations Tetraploidy Genetic marker Chromosomal Translocations Animal Genomics Microsatellite Medicine Karyotypes Departures from Diploidy Genomic Signal Processing Genome Plant Research Article Signal Transduction |
| Zdroj: | PLoS ONE, Vol 16, Iss 10 (2021) PLoS ONE, Vol 16, Iss 10, p e0257100 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Cytogenetic observations, phylogenetic studies and genome analysis using high-density genetic markers have suggested a tetraploid Avena species carrying the C and D genomes (formerly C and A) to be the donor of all hexaploid oats (AACCDD). However, controversy surrounds which of the three extant CCDD tetraploid species—A. insularis, A. magna and A. murphyi—is most closely related to hexaploid oats. The present work describes a comparative karyotype analysis of these three CCDD tetraploid species and two hexaploid species, A. sativa and A. byzantina. This involved the use of FISH with six simple sequence repeats (SSRs) with the motifs CT, AAC, AAG, ACG, ATC and ACT, two repeated ribosomal sequences, and C genome-specific repetitive DNA. The hybridization pattern of A. insularis with oligonucleotide (AC)10 was also determined and compared with those previously published for A. sativa and A. byzantina. Significant differences in the 5S sites and SSR hybridization patterns of A. murphyi compared to the other CCDD species rule out its being directly involved in the origin of the hexaploids. In contrast, the repetitive and SSR hybridization patterns shown by the D genome chromosomes, and by most of the C genome chromosomes of A. magna and A. insularis, can be equated with the corresponding chromosomes of the hexaploids. Several chromosome hybridization signals seen for A. insularis, but not for A. magna, were shared with the hexaploid oats species, especially with A. byzantina. These diagnostic signals add weight to the idea that the extant A. insularis, or a direct ancestor of it, is the most closely related progenitor of hexaploid oats. The similarity of the chromosome hybridization patterns of the hexaploids and CCDD tetraploids was taken as being indicative of homology. A common chromosome nomenclature for CCDD species based on that of the hexaploid species is proposed. |
| Databáze: | OpenAIRE |
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