Activation of anterior thalamic reticular nucleus GABAergic neurons promotes arousal from propofol anesthesia in mice
| Autor: | Tianjie Yuan, Wenxian Li, Nanqi Li, Junli Jiang, Yanjun Liu, Bing Chen, P O Gao, Bingqian Fan, Ying Xia, Yirong Cai, Han Yuan, Weifeng Yu, Yingfu Jiao |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Biophysics Optogenetics Biochemistry gamma-Aminobutyric acid Arousal Mice 03 medical and health sciences 0302 clinical medicine 030202 anesthesiology Animals Medicine Circadian rhythm GABAergic Neurons Propofol Thalamic reticular nucleus business.industry General Medicine medicine.anatomical_structure Anterior Thalamic Nuclei GABAergic Wakefulness business Neuroscience 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Acta Biochimica et Biophysica Sinica. 53:883-892 |
| ISSN: | 1672-9145 |
| Popis: | Propofol is widely used for the induction and maintenance of anesthesia, which causes a rapid loss of consciousness. However, the mechanisms underlying the hypnosis effect of propofol are still not fully understood. The thalamic reticular nucleus (TRN) is crucial for regulating wakefulness, sleep rhythm generation, and sleep stability, while the role of TRN in the process of propofol-induced anesthesia is still unknown. Here, we investigated the function of the anterior TRN in propofol general anesthesia. Our results demonstrated that the neural activity of anterior TRN is suppressed during propofol anesthesia, whereas it is robustly activated from anesthesia by recording the calcium signals using fiber photometry technology. The results showed that the activation of anterior TRN neurons by chemogenetic and optogenetic methods shortens the emergency time without changing the induction time. Conversely, chemogenetic or optogenetic inhibition of the TRN neurons leads to a delay in the recovery time. Our study showed that anterior TRN is crucial for behavioral arousal without affecting the induction time of propofol anesthesia. |
| Databáze: | OpenAIRE |
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