Comparative Sensitivity of Two Tests for the Diagnosis of Multiple Hemoparasite Infection of Cattlea
| Autor: | J. A. Alvarez, Juan Mosqueda, Juan A. Ramos, G. M. Buening, Julio V. Figueroa, G. J. Canto |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Anaplasmosis
animal diseases Cattle Diseases Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology law.invention History and Philosophy of Science law Babesiosis parasitic diseases Multiplex polymerase chain reaction medicine Animals Polymerase chain reaction Babesia bigemina DNA Primers Microscopy biology Inoculation General Neuroscience Babesia bovis medicine.disease biology.organism_classification Virology Blood Cattle Oligonucleotide Probes |
| Zdroj: | Annals of the New York Academy of Sciences. 791:117-127 |
| ISSN: | 0077-8923 |
| DOI: | 10.1111/j.1749-6632.1996.tb53518.x |
| Popis: | The purpose of this study was to evaluate the efficacy of light microscopy (LM) examination of blood smears and a multiplex polymerase chain reaction (MPCR) assay, in terms of their ability to detect cattle experimentally infected with Babesia bovis, Babesia bigemina, and Anaplasma marginale. Blood samples were collected from 32 intact, 1-2 year old, Holstein bulls, previous to and after simultaneous inoculation of culture-derived or field isolates of B. bovis- and B. bigemina-infected erythrocytes. To establish the triple hemoparasite infection, 16 of the bulls were also inoculated with a calf-derived isolate of A. marginale. The results showed that both tests had 100% specificity. In contrast, the sensitivities of the MPCR assay against the LM test were 93.5% and 70.9%; 96.7% and 100%; and 93.8% and 93.8% for B. bovis, B. bigemina, and A. marginale infection, respectively. The advantages and disadvantages of the MPCR assay to differentially diagnose cattle with multiple hemoparasite infection are discussed. |
| Databáze: | OpenAIRE |
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