High expression of long non-coding RNA ZEB1-AS1 promotes colorectal cancer cell proliferation partially by suppressing p15 expression
| Autor: | Zhuyin Qian, Jin Zhu, Huangbo Gong, Hao Wen, Xiaojun Yang, Xuhui Zhu, Yifan Lian |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Small interfering RNA Epithelial-Mesenchymal Transition Colorectal cancer Apoptosis Kaplan-Meier Estimate Biology Mice 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor Biomarkers Tumor medicine Animals Humans RC254-282 Aged Cell Proliferation Cyclin-Dependent Kinase Inhibitor p15 Cell growth Neoplasms. Tumors. Oncology. Including cancer and carcinogens General Medicine Middle Aged Cell cycle medicine.disease Xenograft Model Antitumor Assays Long non-coding RNA Gene Expression Regulation Neoplastic Transplantation 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Heterografts Female RNA Long Noncoding Colorectal Neoplasms |
| Zdroj: | Tumor Biology, Vol 39 (2017) |
| ISSN: | 1423-0380 1010-4283 |
| DOI: | 10.1177/1010428317705336 |
| Popis: | This study aims to investigate the function of long non-coding RNA ZEB1-AS1, reveal its molecular mechanism in colorectal cancer cell growth, and evaluate its clinical significance in colorectal cancer patients. ZEB1-AS1 has reported in the development of several cancers, but the biological role of it in colorectal cancer has not been discussed. In this report, ZEB1-AS1 expression level was measured with quantitative real-time polymerase chain reaction in 63 pairs of colorectal cancer tissues and paired adjacent non-tumor colorectal tissues. The relationship between ZEB1-AS1 expression and overall survival was analyzed by virtue of Kaplan-Meier analysis. Subsequently, small interfering RNA or lentivirus vector-mediated lncRNA ZEB1-AS1 was transfected into colorectal cancer cell lines. Cell viability and apoptosis were examined. Later, nude mouse transplantation experiment was conducted to evaluate the effect of ZEB1-AS1 on colorectal cancer development in vivo. It turns out that ZEB1-AS1 is upregulated in colorectal cancer tissues and its expression is significantly associated with overall survival rate and recurrence-free survival. Upregulation of ZEB1-AS1 colorectal cancer promotes cell proliferation and inhibits cell apoptosis. In addition, cell cycle inhibitory protein p15 participates in the oncogenic function of ZEB1-AS1. Collectively, ZEB1-AS1 has asignificant effect on colorectal cancer pathological process and serves as a valuable prognostic biomarker for colorectal cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|