Mesorhizobium ventifaucium sp. nov. and Mesorhizobium escarrei sp. nov., two novel root-nodulating species isolated from Anthyllis vulneraria
| Autor: | Roba, Mohamad, Anne, Willems, Antoine, Le Quéré, Marjorie, Pervent, Géraldine, Maynaud, Maurine, Bonabaud, Emeric, Dubois, Jean-Claude, Cleyet-Marel, Brigitte, Brunel |
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| Rok vydání: | 2022 |
| Předmět: |
DNA-DNA HYBRIDIZATION
DNA Bacterial DIVERSITY Rhizobia Mesorhizobium sp.nov DNA Ribosomal Applied Microbiology and Biotechnology Microbiology CLASSIFICATION Soil Symbiovar RNA Ribosomal 16S PLANT Symbiosis Phylogeny Ecology Evolution Behavior and Systematics 16S RIBOSOMAL-RNA Mesorhizobium Biology and Life Sciences Nucleic Acid Hybridization Sequence Analysis DNA TAXONOMY Bacterial Typing Techniques LEGUMES Genes Bacterial Lotus RHIZOBIAL STRAINS MULTILOCUS SEQUENCE-ANALYSIS Root Nodules Plant |
| Zdroj: | SYSTEMATIC AND APPLIED MICROBIOLOGY |
| ISSN: | 0723-2020 1618-0984 |
| DOI: | 10.1016/j.syapm.2022.126341 |
| Popis: | Ten mesorhizobial strains isolated from root-nodules of Anthyllis vulneraria by trapping using soils from southern France were studied to resolve their taxonomy. Their 16S rDNA sequences were identical and indicated that they are affiliated to the genus Mesorhizobium within the group M. prunaredense/M. delmotii/M. temperatum/M. mediterraneum/M. wenxiniae and M. robiniae as the closest defined species. Their evolutionary relationships with validated species were further characterized by multilocus sequence analysis (MLSA) using 4 protein-coding housekeeping genes (recA, atpD, glnII and dnaK), that divides the strains in two groups, and suggest that they belong to two distinct species. These results were well-supported by MALDI-TOF mass spectrometry analyses, wet-lab DNA-DNA hybridization (≤58%), and genome-based species delineation methods (ANI 96%, in silico DDH 70%), confirming their affiliation to two novel species. Based on these differences, Mesorhizobium ventifaucium (STM4922 |
| Databáze: | OpenAIRE |
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