In the Intestinal Mucosa of Children With Potential Celiac Disease IL-21 and IL-17A are Less Expressed than in the Active Disease
| Autor: | Valentina Discepolo, D. Ponticelli, K. Ferrara, Merlin Nanayakkara, Mariantonia Maglio, Giuliana Lania, Carmen Gianfrani, Melissa Borrelli, Renata Auricchio, Delia Zanzi, Serena Vitale, Rosita Aitoro, Riccardo Troncone, Maria Vittoria Barone |
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| Přispěvatelé: | Borrelli, Melissa, Gianfrani, Carmela, Lania, Giuliana, Aitoro, Rosita, Ferrara, K, Nanayakkara, Merlin, Ponticelli, D, Zanzi, Delia, Discepolo, Valentina, Vitale, Serena, Barone, MARIA VITTORIA, Troncone, Riccardo, Auricchio, Renata, Maglio, Mariantonia |
| Rok vydání: | 2014 |
| Předmět: |
Male
Duodenum Disease 03 medical and health sciences 0302 clinical medicine Intestinal mucosa mucosal immunology Active disease IL-21 medicine Humans Intestinal Mucosa Child Cells Cultured Hepatology business.industry Interleukins Interleukin-17 Gastroenterology nutritional and metabolic diseases Interleukin digestive system diseases Celiac Disease medicine.anatomical_structure Child Preschool Immunology 030211 gastroenterology & hepatology Female Interleukin 17 business 030215 immunology |
| Zdroj: | The American journal of gastroenterology 111 (2016). doi:10.1038/ajg.2015.390 info:cnr-pdr/source/autori:Borrelli M, Gianfrani C, Lania G, Aitoro R, Ferrara K, Nanayakkara M, Ponticelli D, Zanzi D, Discepolo V, Vitale S, Troncone R, Auricchio R, and Maglio M/titolo:In the intestinal mucosa of children with potential coeliac disease IL-21 and IL-17a are less expressed than in the active disease./doi:10.1038%2Fajg.2015.390/rivista:The American journal of gastroenterology/anno:2016/pagina_da:/pagina_a:/intervallo_pagine:/volume:111 |
| ISSN: | 1572-0241 |
| DOI: | 10.1038/ajg.2015.390 |
| Popis: | OBJECTIVES: Potential celiac disease (CD) patients are at an increased risk to developing CD as indicated by positive CD-associated serology. We investigated in duodenal mucosa of such patients the presence of both IL-21 and IL-17A and the role of gliadin peptides and IL-15 in their expression. METHODS: Duodenal biopsies from 76 active CD, 90 potential CD, and 58 control patients were analyzed for IL-21 and/or IL-17A production by quantitative real-time PCR, immunohistochemistry, flow cytometry, and ELISA. The presence of IL-21 receptor was investigated by western blot. Potential CD duodenal fragments were cultured with gliadin peptides (PTG) and/or IL-15 and the expression/production of IL-21 and IL-17A assessed by quantitative real-time PCR and by immunohistochemistry. RESULTS: In potential CD, IL-21 was lower than in active CD, in terms of RNA expression (P |
| Databáze: | OpenAIRE |
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