Identification and genetic characterization of equine infectious anemia virus in Western Balkans

Autor: Nicolas Berthet, Aymeric Hans, Sara Savić, Diana Lupulović, Živoslav Grgić, D. Gaudaire, Kazimir Matović, Alexandre Deshiere
Přispěvatelé: Scientific Veterinary Institute 'Novi Sad', Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Pasteur de Shanghai, Académie des Sciences de Chine - Chinese Academy of Sciences (IPS-CAS), Réseau International des Instituts Pasteur (RIIP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris], This study was supported by the Hubert Curien Partnerships (PHC-Pavle Savic), the European Reference Laboratory for Equine Diseases other than African Horse Sickness, ANSES’s own institutional resources and the Ministry of Science and Technological Development of the Republic of Serbia (Grant no. TR31071). Alexandre Deshiere and 'CENTAURE project' was supported by a grant awarded by the Regional Council of Normandy and the French Ministry of Higher Education, within the framework of CPER 2015–2020 and FEDER/FSE 2014–2020. Nicolas Berthet is also supported by the Chinese Academy of Sciences and a Shanghai Municipal Science and Technology Major Project (Grant No. 2019SHZDZX02). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication., The authors thank the sequencing platform SeSAME of the Centre François Baclesse in Caen, Normandy and official veterinary services in Serbia. Laboratoire de santé animale, site de Normandie is a member of the GIS Centaure equine research and authors thanks Regional Council of Normandy, France for providing an excellent scientific environment, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité)
Jazyk: angličtina
Rok vydání: 2021
Předmět:
040301 veterinary sciences
Veterinary medicine
[SDV]Life Sciences [q-bio]
viruses
Population
Enzyme-Linked Immunosorbent Assay
Genome
Viral
Virus
Serology
0403 veterinary science
Equine infectious anemia
03 medical and health sciences
Seroepidemiologic Studies
SF600-1100
AGID test
Animals
MESH: Animals
Horses
education
MESH: Horses
MESH: Phylogeny
Phylogeny
030304 developmental biology
Whole genome sequencing
0303 health sciences
education.field_of_study
MESH: Seroepidemiologic Studies
General Veterinary
biology
Horse
MESH: Enzyme-Linked Immunosorbent Assay
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Ouchterlony double immunodiffusion
Virology
3. Good health
MESH: Serbia
MESH: Equine Infectious Anemia
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Equine Infectious Anemia
NGS
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: Infectious Anemia Virus
Equine
Viral disease
MESH: Genome
Viral
Serbia
Research Article
Infectious Anemia Virus
Equine
Zdroj: BMC Veterinary Research
BMC Veterinary Research, BioMed Central, 2021, 17 (1), pp.168. ⟨10.1186/s12917-021-02849-2⟩
BMC Veterinary Research, Vol 17, Iss 1, Pp 1-6 (2021)
BMC Veterinary Research, 2021, 17 (1), pp.168. ⟨10.1186/s12917-021-02849-2⟩
ISSN: 1746-6148
Popis: Background Equine infectious anemia (EIA) is a viral disease, caused by the Equine Infectious Anemia virus (EIAV) belonging to the Retroviridae family, genus Lentivirus. Horses (or equids) infected with EIAV are lifelong carriers and they remain contagious for other horses even in the absence of clinical signs. So far, EIAV infection has been reported among horses in North and South America, France, Germany, Italy, Hungary and Romania, with no publication regarding the presence of EIAV in horses in Serbia. To determine the circulation of EIAV among, approximately, the 5000 horses of the Vojvodina region, northern part of Serbia, 316 serum undergone serological testing for EIA. Then, identification and full genome sequencing using next generation sequencing was performed from one EIA positive horse. Results the 316 sera were tested with 3 different commercial agar gel immunodiffusion (AGID) tests and two different commercial enzyme-linked immunosorbent assay (ELISA). With the three AGID kits, 311 (98.4%) among the 316 tested sera were negative and only five (1.6%) sera were positive for EIA. Some discrepancies were seen for the two ELISA kits tested since one exhibited the same results as AGID test and the second gave 295 sera with negative results, five with a positive result and 16 with doubtful outcome. Phylogenetic analysis performed using the full genome sequence showed that EIAV characterized from a horse in Serbia is different from those identify so fare around the world and form a distinct and separate group together with another EIAV strain. Conclusions This study demonstrate for the first time that EIAV is circulating at a low level in the horse population from the Northern part of Serbia. Interestingly, phylogenetic data indicates that this EIAV from the western Balkan region of Europe belongs to a new cluster.
Databáze: OpenAIRE
Externí odkaz: