Human T Helper Cell Epitopes Overlap B Cell and Putative Cytotoxic T Cell Epitopes in the E2 Protein of Human Papillomavirus Type 16
| Autor: | G. Stellato, Jorma Paavonen, T. Kuoppala, Matti Lehtinen, M.H. Hibma |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Biophysics Uterine Cervical Neoplasms Biology Lymphocyte Activation Biochemistry Epitope Viral Proteins Interleukin 21 medicine Humans Cytotoxic T cell Amino Acid Sequence Antigen-presenting cell Papillomaviridae Molecular Biology B cell Linear epitope Carcinoma Histocompatibility Antigens Class I Histocompatibility Antigens Class II Oncogene Proteins Viral T-Lymphocytes Helper-Inducer Cell Biology T helper cell Uterine Cervical Dysplasia Virology Molecular biology DNA-Binding Proteins medicine.anatomical_structure Epitope mapping Female Epitope Mapping T-Lymphocytes Cytotoxic |
| Zdroj: | Biochemical and Biophysical Research Communications. 209:541-546 |
| ISSN: | 0006-291X |
| Popis: | Activation of T helper cells is a prerequisite for the function of cytotoxic T lymphocytes (CTL) and the maturation of the B cell response. Because epitopes recognized by each of these cells may overlap, we scanned the E2 protein of human papillomavirus (HPV) type 16 to identify the T helper cell epitopes. Four major T helper cell epitopes (mapping between aa:s 11-25, 141-155, 191-205 & 231-245) and adjacent human B cell epitopes were found. The first peptide-defined epitope (RLNV) 11CQDKILTHYENDSTD25 overlapped five putative HLA-I (A1, A2, A0205, A3 & A11) binding motifs (CQDKILTHY, RLNVCQDKI, NVCQDKIL, RLNVCQDK & RLNVCQDK). This epitope is also part of an N-terminal alpha-helix which may form four HLA-II (DR2, DR4, DR7 & DR8) specific agretopes for structures recognizable by the T cell receptor (e.g. KILT). The second epitope 141EEASVTVVEGOVDYY155 (GLYY) overlapped the putative HLA-A1 & HLA-Bw37 binding motifs (VVEGQVDYY/QVDYYGLYY and EEASVTVV), and two HLA-II (DR1 & DR3) specific agretopes. The third and fourth epitopes were not associated with more than one putative CTL epitope each. Only the first epitope shared considerable aa-homology with corresponding regions of other genital HPV types. |
| Databáze: | OpenAIRE |
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