In situ localization of human papilloma virus type 16 DNA in a metastasis of an endocervical adenocarcinoma
| Autor: | H. E. Fokke, J. Ter Schegget, J. van der Noordaa, M. Polak, H. Volkers, J. Barents, J. M. M. Walboomers, H. J. Houthoff |
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| Rok vydání: | 1987 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Uterine Cervical Neoplasms In situ hybridization Biology Adenocarcinoma DNA sequencing Virus Metastasis chemistry.chemical_compound Stroma Virology medicine Humans Papillomaviridae Southern blot Pelvic Neoplasms virus diseases Nucleic Acid Hybridization medicine.disease Molecular biology Tumor Virus Infections Infectious Diseases chemistry DNA Viral Female Molecular probe DNA |
| Zdroj: | Intervirology. 27(2) |
| ISSN: | 0300-5526 |
| Popis: | Metastatic tissue from an endocervical adenocarcinoma was examined for human papilloma virus (HPV) DNA sequences. Southern blot analysis with 32P-labeled viral probes revealed the presence of about 150 copies of HPV-16 DNA per cellular genome. DNA in situ hybridization of frozen sections with biotinylated probes showed that HPV-16 DNA was exclusively detectable in the nuclei of the malignant epithelial cells and not in the stroma. |
| Databáze: | OpenAIRE |
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