Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus
| Autor: | Seng-Meng Phang, Chai-Yaw Teo, Victor Wong Thi Wong, Evelyn Lo |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
DNA polymerase
Molecular Sequence Data Restriction Mapping Bacillus Polymerase Chain Reaction DNA sequencing law.invention Geobacillus stearothermophilus Plasmid law Genetics Genomic library Amino Acid Sequence Cloning Molecular Gene Klenow fragment Genomic Library Base Sequence Sequence Homology Amino Acid biology DNA Polymerase II General Medicine DNA Polymerase I Molecular biology genomic DNA Genes Bacterial biology.protein Recombinant DNA Genome Bacterial Plasmids |
| Zdroj: | Gene. 163:65-68 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/0378-1119(95)00387-l |
| Popis: | A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme. |
| Databáze: | OpenAIRE |
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