Free fatty acid levels in fluid of dominant follicles at the preferred insemination time in dairy cows are not affected by early postpartum fatty acid stress
| Autor: | Aardema, Hilde, Gadella, Bart M, van de Lest, Chris H A, Brouwers, Jos F H M, Stout, Tom A E, Roelen, Bernard A J, Vos, Peter L A M, LS GZ Landbouwhuisdieren, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, LS Veterinaire biochemie, Sub MS-faciliteit, LS Voortplanting Inwendige Ziekten, Sub Celbiologisch lab., ES/FAH BRC, Fertility & Reproduction |
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| Přispěvatelé: | LS GZ Landbouwhuisdieren, LS Klinische Reproductie, Sub Biologie van de mannelijke gameet, Sub Reproductie mannelijk, LS Equine Muscoskeletal Biology, LS Veterinaire biochemie, Sub MS-faciliteit, LS Voortplanting Inwendige Ziekten, Sub Celbiologisch lab., ES/FAH BRC, Fertility & Reproduction |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Ovulation
medicine.medical_specialty media_common.quotation_subject Fatty Acids Nonesterified Insemination chemistry.chemical_compound Stress Physiological Internal medicine Follicular phase Genetics medicine Animals postpartum Progesterone Retrospective Studies media_common chemistry.chemical_classification Principal Component Analysis Estradiol dairy cow Postpartum Period Fatty acid Metabolism Follicular fluid follicular fluid Oleic acid Endocrinology chemistry Cattle Female Animal Science and Zoology lipids (amino acids peptides and proteins) Stearic acid Energy Metabolism Stearic Acids Oleic Acid free fatty acid Food Science |
| Zdroj: | Journal of Dairy Science, 98(4), 2322. Elsevier Limited |
| ISSN: | 0022-0302 |
| Popis: | The fertility of high-yielding dairy cows has declined during the last 3 decades, in association with a more profound negative energy balance (NEB) during the early weeks postpartum. One feature of this NEB is a marked elevation in circulating free fatty acid (FFA) concentrations. During the early postpartum period (≤d 42), circulatory FFA levels were measured weekly, and progesterone concentrations and the diameter of the dominant follicles were determined thrice weekly. Retrospectively, cows that ovulated within 35 d postpartum were grouped as "normal ovulating" cows (n=5), and the others were grouped as "delayed ovulating" cows (n=5). In both groups, high total FFA levels (>500 µ M ) were evident immediately postpartum. Interestingly, cows with delayed ovulation had higher plasma FFA concentrations in the first weeks postpartum compared with normal ovulating cows. In both cow groups, FFA decreased to control levels of non-NEB cows within 3 wk postpartum. The FFA compositions and concentrations in fluids from the dominant follicles of postpartum cows were not different between the normal and delayed ovulating cows when measured at potential insemination points: d 55, 80, and 105 postpartum. Interestingly, the concentration of monounsaturated oleic acid was higher and that of saturated stearic acid lower in follicular fluids of both groups compared with that in blood. The level of FFA in follicular fluid was correlated with the ratio of 17β-estradiol (E 2 ) to progesterone (P 4 ) in follicular fluid, with a relatively high level of unsaturated FFA in follicles with a low E 2 :P 4 ratio. Taken together, these results indicate that a more severe NEB early postpartum is related to a delay in the first postpartum ovulation and does not affect FFA composition in follicular fluid at the preferred insemination time. The high FFA level in dominant follicles with a low E 2 :P 4 ratio may be due to a different FFA metabolism in these follicles. The diagnostic value of this observation for selective screening of dominant follicles needs further investigation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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