Comprehensive and computational analysis of genes in human umbilical vein endothelial cells responsive to X-irradiation
| Autor: | Toshiyasu Iwasaki, Yuichi Hattori, Takashi Kondo, Takaharu Nomura, Yukihiro Furusawa, Yoshiaki Tabuchi, Qing-Li Zhao |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ionizing radiation lcsh:QH426-470 Microarray Gene regulatory network Inflammation Biology Biochemistry Umbilical vein 03 medical and health sciences 0302 clinical medicine Interferon Genetics medicine Gene Human umbilical endothelial cells Computational gene Inflammatory response Regular Article Cardiovascular disease lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine IRF7 medicine.symptom Biotechnology medicine.drug |
| Zdroj: | Genomics Data Genomics Data, Vol 8, Iss C, Pp 126-130 (2016) |
| ISSN: | 2213-5960 |
| Popis: | Radiation exposure such as A-bomb or radiation therapy is considered a major health-risk factor for cardiovascular disease. In order to understand the molecular mechanisms underlying the inflammatory reaction frequently encountered in the vascular system after exposure to ionizing radiation, we carried out a global scale microarray and computational gene expression analyses on human umbilical endothelial cells (HUVECs) exposed to X-ray (2.5Gy). The gene ontology analysis revealed that the down-regulated genes were associated with cell cycle regulation, whereas the up-regulated genes were associated with inflammatory responses, in particular, the type 1 interferon response. The computational analysis using ingenuity pathway analysis also identified a gene network containing the interferon response factor 7 (IRF7) and its transcriptional targets such as interferon-induced transcripts (IFITs) and Mx1, which have been known to be associated with inflammation in endothelial cells. The up-regulated genes and the gene network identified here may explain the inflammatory response induced by X-irradiation. These findings uncover part of the molecular basis of the mechanism(s) of the inflammatory disorder in response to X-irradiation in HUVECs. The dataset is publicly available at the Gene Expression Omnibus (GEO) repository (http://www.ncbi.nlm.nih.gov/geo/) with accession number GSE76484. |
| Databáze: | OpenAIRE |
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