Ovarian monocyte progenitor cells: phenotypic and functional characterization
| Autor: | Margi Baldwin, Nagwa El-Badri, Wilfredo Chamizo, Danika Lerner, Cherry J Pascual, Soichi Haraguchi, Paul R. Sanberg |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Stromal cell
Cell Transplantation Population Green Fluorescent Proteins Lewis X Antigen Mice Transgenic Biology Methylcellulose Monocytes Immunophenotyping Mice medicine Cell Adhesion Leukocytes Animals Cell Lineage Progenitor cell education Cells Cultured Cell Proliferation education.field_of_study Mice Inbred BALB C CD11b Antigen Monocyte Macrophages Stem Cells Ovary Cell Biology Hematology Alkaline Phosphatase Flow Cytometry Embryonic stem cell Cell biology CD11c Antigen DNA-Binding Proteins Mice Inbred C57BL Haematopoiesis Microscopy Electron Proto-Oncogene Proteins c-kit medicine.anatomical_structure Phenotype Leukocyte Common Antigens Female Stem cell Octamer Transcription Factor-3 Developmental Biology Transcription Factors |
| Zdroj: | Stem cells and development. 14(2) |
| ISSN: | 1547-3287 |
| Popis: | Leukocytes of the macrophage lineage are abundant in the ovarian tissues and have an important function in both follicular development and regression of postovulatory follicles. In this study, we tested the hypothesis that continuous production of macrophages in the ovarian stroma is maintained by a resident population of progenitors. We established a long-term culture of ovarian follicular stromal cells from BALB/c and green fluorescent protein-transgenic (GFP-TG) C57BL/6 mice. Nonadherent cells were collected and tested for hematopoietic function in vitro and in vivo. Histological and ultrastructural analyses revealed a homogenous population of monocyte-like rounded cells. Nonadherent cells continued to proliferate in culture for several months without senescence. When plated at very low density in methylcellulose, these cells formed colonies consisting of monocyte-like cells. Ovarian monocyte-like cells reacted with CD45, CD11b, CD11c, and Ly6-Gr-1 cell surface markers. A distinct CD45low population within these cells reacted with CD117 (C-kit) surface marker, suggestive of a primitive hematopoietic progenitor. Fifty thousand nonadherent cells failed to provide radioprotection to lethally irradiated mice and thus were not considered to be equivalent to pluripotent hematopoietic stem cells. Ovarian nonadherent stromal cells were positive for alkaline phosphatase but lacked embryonic cell antigens stage-specific embryonic antigen (SSEA-1) and Oct-4. We conclude that in the ovaries, a higher requirement for macrophages is provided by a resident stromal population of progenitors whose progeny is restricted to the production of cells of the monocyte-macrophage lineage. |
| Databáze: | OpenAIRE |
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