Optimization of nutritional components of medium by response surface methodology for enhanced production of lactase
| Autor: | K. Vidya Prabhakar, T.C. Venkateswarulu, R. Bharath Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Central composite design medicine.medical_treatment Tryptophan Lactase Environmental Science (miscellaneous) Biology 01 natural sciences Agricultural and Biological Sciences (miscellaneous) Lactase activity 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry 010608 biotechnology medicine Yeast extract Fermentation Original Article Food science Response surface methodology Lactose Biotechnology |
| Popis: | Lactase has excellent applications in dairy industry and commercially this enzyme is produced from bacterial sources but not in high yields. In this work, the production of lactase was improved by designing of nutrient components in fermentation medium by one factor at a time. Lactose and yeast extract were selected as preferable carbon and nitrogen sources for lactase production with tryptophan and MgSO4 showing enhanced production. Statistical analysis proved to be a useful and powerful tool in developing optimum fermentation conditions. The individual and interactive role of lactose, yeast extract, magnesium sulfate, and tryptophan concentration on lactase production was examined by central composite design. Submerged fermentation with Bacillus subtilis strain VUVD001 produced lactase activity of 63.54 U/ml in optimized medium. The activity was threefold higher in comparison to an unoptimized medium. This result confirmed that the designed medium was useful for producing higher yields of lactase. |
| Databáze: | OpenAIRE |
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