The PCP Protein Vangl2 Regulates Migration of Hindbrain Motor Neurons by Acting in Floor Plate Cells, and Independently of Cilia Function
Autor: | Derrick M. Glasco, Michael P. Matise, Peng Huang, Hui Wang, Suman Gurung, Shike Li, Koichi Kawakami, Xiufang Pan, Anand Chandrasekhar, Vinoth Sittaramane, Anagha S. Bock |
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Jazyk: | angličtina |
Rok vydání: | 2013 |
Předmět: |
Cell type
Neurogenesis Rhombomere Kruppel-Like Transcription Factors Hindbrain Biology Zinc Finger Protein Gli2 Article 03 medical and health sciences Mice 0302 clinical medicine Cell Movement Cell polarity Animals Cilia Zebrafish Molecular Biology 030304 developmental biology Floor plate Motor Neurons 0303 health sciences Cilium Cell Polarity Gene Expression Regulation Developmental Membrane Proteins Anatomy Cell Biology Zebrafish Proteins biology.organism_classification Cell biology Rhombencephalon nervous system 030217 neurology & neurosurgery Developmental Biology |
Popis: | Vangl2, a core component of the Planar Cell Polarity pathway, is necessary for the caudal migration of Facial Branchiomotor (FBM) neurons in the vertebrate hindbrain. Studies in zebrafish suggest that vangl2 functions largely non-cell autonomously to regulate FBM neuron migration out of rhombomere 4 (r4), but the cell-type within which it acts is not known. Here, we demonstrate that vangl2 functions largely in floor plate cells to regulate caudal neuronal migration. Furthermore, FBM neurons fail to migrate caudally in the mouse Gli2 mutant that lacks the floor plate, suggesting an evolutionarily conserved role for this cell type in neuronal migration. Although hindbrain floor plate cilia are disorganized in vangl2 mutant embryos, cilia appear to be dispensable for neuronal migration. Notably, Vangl2 is enriched in the basolateral, but not apical, membranes of floor plate cells. Taken together, our data suggest strongly that Vangl2 regulates FBM neuron migration by acting in floor plate cells, independently of cilia function. |
Databáze: | OpenAIRE |
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