A drosophila genetic model of nephrolithiasis: transcriptional changes in response to diet induced stone formation
| Autor: | Benjamin W. Turney, Vera Y. Chung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Malpighian tubule system Candidate gene Pathology medicine.medical_specialty Transcription Genetic Urology 030232 urology & nephrology Malpighian Tubules lcsh:RC870-923 Nephrolithiasis Pathogenesis 03 medical and health sciences 0302 clinical medicine Transcription (biology) Genetic model medicine Animals Gene 2. Zero hunger Models Genetic business.industry Oxalic Acid fungi RNA General Medicine lcsh:Diseases of the genitourinary system. Urology Cell biology Diet 030104 developmental biology Reproductive Medicine Drosophila RNA extraction business Research Article |
| Zdroj: | BMC Urology BMC Urology, Vol 17, Iss 1, Pp 1-9 (2017) |
| ISSN: | 1471-2490 |
| Popis: | Background Urolithiasis is a significant healthcare issue but the pathophysiology of stone disease remains poorly understood. Drosophila Malpighian tubules were known to share similar physiological function to human renal tubules. We have used Drosophila as a genetic model to study the transcriptional response to stone formation secondary to dietary manipulation. Methods Wild-type male flies were raised on standard medium supplemented with lithogenic agents: control, sodium oxalate (NaOx) and ethylene glycol (EG). At 2 weeks, Malpighian tubules were dissected under polarized microscope to visualize crystals. The parallel group was dissected for RNA extraction and subsequent next-generation RNA sequencing. Results Crystal formation was visualized in 20%(±2.2) of flies on control diet, 73%(±3.6) on NaOx diet and 84%(±2.2) on EG diet. Differentially expressed genes were identified in flies fed with NaOx and EG diet comparing with the control group. Fifty-eight genes were differentially expressed (FDR |
| Databáze: | OpenAIRE |
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