Ionizing radiation induced long-term alterations in the adult rat rostral migratory stream
| Autor: | Jan Lehotsky, Marian Adamkov, Eva Hajtmanová, Ria Trylčová, Sona Balentova |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Doublecortin Domain Proteins
Male Doublecortin Protein Histology Rostral migratory stream Neurogenesis Subventricular zone Biology Cerebral Ventricles Ionizing radiation Prosencephalon Neuroblast Cell Movement medicine Animals Rats Wistar Neurons CD11b Antigen Microglia Neuropeptides Cell Biology General Medicine Anatomy Doublecortin Radiation Injuries Experimental Phenotype medicine.anatomical_structure nervous system Gamma Rays biology.protein Immunohistochemistry Microtubule-Associated Proteins |
| Zdroj: | Acta Histochemica. 116:265-271 |
| ISSN: | 0065-1281 |
| DOI: | 10.1016/j.acthis.2013.08.002 |
| Popis: | Ionizing radiation can induce significant injury to normal brain structures. To assess radiation-induced late effects, adult male Wistar rats received whole-body exposure with fractionated doses of gamma rays (a total dose of 4 Gy) and were investigated thirty, sixty and ninety days later. Immunohistochemistry and confocal microscopy were used to determine the density of neuroblasts derived from the anterior subventricular zone (SVZa) and brain resident microglia distributed along and/or adjacent to subventricular zone–olfactory bulb axis (SVZ–OB axis). Cell counting was performed in four anatomical parts along the well defined pathway, known as the rostral migratory stream (RMS) represented by the SVZa, vertical arm, elbow and horizontal arm of the RMS. Strong overdistribution of neuroblasts was seen in the SVZa thirty and sixty days after irradiation replaced by a steep decline in the following parts of the RMS and the highest decrease ninety days after radiation treatment along the entire SVZ–OB axis. Radiation treatment led to a decline or loss of microglia in almost all counted parts through the entire experiment. Results showed that ultimate decline of the SVZa descendants and loss of microglia suggests a contributory role of reduced neurogenesis in the development of radiation-induced late effects. |
| Databáze: | OpenAIRE |
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