Genetic Characteristics and Pathogenicity of a Novel Porcine Deltacoronavirus Southeast Asia-Like Strain Found in China
| Autor: | Ping Zhao, Kang Ouyang, Ying Lu, Hejie Wang, Zhao Wu, Chen Zhongwei, Tingting Yuan, Qin Yibin, Weijian Huang, Ying He, Xue Mi, Cui Yang, Chunjie Yang, Ying Chen, Zhuyue Wu, Zuzhang Wei, Xianfeng Yi, Lu Bingxia |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Whole genome sequencing Genetic diversity virus isolation General Veterinary Strain (chemistry) Accession number (library science) Veterinary medicine 030106 microbiology diarrhea Biology Brief Research Report piglets Southeast asian Virology 03 medical and health sciences Titer 030104 developmental biology GenBank SF600-1100 pathogenicity Veterinary Science Gene genomic characteristics porcine deltacoronavirus |
| Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 8 (2021) |
| ISSN: | 2297-1769 |
| Popis: | Farmers involved in the lucrative pork trading business between China and Southeast Asian countries should be aware of a recently discovered novel porcine deltacoronavirus (PDCoV) in Guangxi province, China. A PDCoV strain, CHN/GX/1468B/2017, was isolated from the small intestinal contents of piglets with diarrhea from this region, with a titer of 1 × 108.0 TCID50/mL on LLC-PK cells. The full-length genome sequence consists of 25,399 nt as determined by next-generation sequencing and this was deposited in the GenBank (accession number MN025260.1). Genomic analysis showed that CHN/GX/1468B/2017 strain had 96.9~99.4% nucleotide homology with other 87 referenced PDCoV strains from different areas, and contained 6 and 9-nt deletions at positions 1,733~1,738 and 2,804~2,812, respectively, in the ORF1a gene. Phylogenetic analyses based on the whole gene sequence as well as S protein and ORF1a/1b protein sequences all showed that this strain was closely related to the Southeast Asia strain. When 7-day-old piglets were inoculated orally with the CHN/GX/1468B/2017 strain, they developed severe diarrhea, with a peak of fecal viral shedding at 4 days post-infection. Although no death or fever were observed, the CHN/GX/1468B/2017 strain produced a wide range of tissue tropism, with the main target being the intestine. Importantly, the VH:CD ratios of the jejunum and ileum in infected piglets were significantly lower than controls. These results indicate that CHN/GX/1468B/2017, isolated in China, is a novel PDCoV Southeast Asia-like strain with distinct genetic characteristics and pathogenicity. This finding enriches the international information on the genetic diversity of PDCoV. |
| Databáze: | OpenAIRE |
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