NgR expression in macrophages promotes nerve regeneration after spinal cord injury in rats
| Autor: | Xiang Guo, Guodong Yin, Fengjin Zhou, Bin Ni, Jun Liu, Zhuangchen Zhu, Qunfeng Guo |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Cord Phagocytosis Receptors Cell Surface GPI-Linked Proteins Rats Sprague-Dawley Western blot Nogo Receptor 1 medicine Animals Macrophage Orthopedics and Sports Medicine Receptor Spinal cord injury Cells Cultured Spinal Cord Injuries medicine.diagnostic_test business.industry Macrophages Regeneration (biology) General Medicine Anatomy medicine.disease Molecular biology In vitro Nerve Regeneration Rats Surgery business Myelin Proteins |
| Zdroj: | Archives of Orthopaedic and Trauma Surgery. 130:945-951 |
| ISSN: | 1434-3916 0936-8051 |
| DOI: | 10.1007/s00402-010-1065-8 |
| Popis: | This study aimed to investigate the expression of Nogo-66 receptor (NgR) in macrophages after SCI and clarify its role in neuron regeneration. Macrophages harvested from injured spine cord of rats were stained by double immunofluorescence labeling technique to observe the expression of NgR at histological and cellular levels. Macrophages which expressed NgR were constructed in vitro, and then the effects of NgR on macrophage phagocytosis and neuraxon regeneration in three groups (NgR-macrophages group, mock group and normal macrophages group) were studied using Western blot, micro-MTT colorimetry, and LDH assay separately. The results showed that CD68-positive macrophages in injured tissue of spine cord expressed NgR after double immunofluorescence staining on day 7 after SCI, and so did macrophages isolated and cultured from the injured spine cord. The results of Western blot showed that phagocytosis of macrophages in NgR-macrophages group was much better than that in mock group and normal macrophage group (p |
| Databáze: | OpenAIRE |
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