The effect of baicalein on endoplasmic reticulum stress and autophagy on liver damage
| Autor: | Cihan Tanrikut, Emre Entok, Derya Ustuner, Dilek Burukoglu, Umut Kerem Kolac, Mehmet Cengiz Ustuner, Z. Ozdemir Koroglu |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Health Toxicology and Mutagenesis ATG5 Toxicology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Autophagy Animals Rats Wistar biology Carbon Tetrachloride Poisoning ATF6 Endoplasmic reticulum ATF4 General Medicine Endoplasmic Reticulum Stress biology.organism_classification Molecular biology Rats Baicalein 030104 developmental biology chemistry 030220 oncology & carcinogenesis Flavanones Unfolded protein response Scutellaria baicalensis Female Chemical and Drug Induced Liver Injury Biomarkers |
| Zdroj: | Human & Experimental Toxicology. 40:1624-1633 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | Carbon tetrachloride (CCl4) is a toxic chemical that causes liver injury. CCl4 triggers endoplasmic reticulum (ER) stress and unfolded protein response (UPR). UPR triggers autophagy to deal with the damage. The aim of this study was to investigate the effect of baicalein, derived from Scutellaria baicalensis, on CCl4-induced liver damage concerning ER stress and autophagy. Two groups of Wistar albino rats (n = 7/groups) were treated with 0.2 ml/kg CCl4 for 10 days with and without baicalein. Histological and transmission electron microscopy (TEM) analysis, autophagy, and ER stress markers measurements were carried out to evaluate the effect of baicalein. Histological examinations showed that baicalein reduced liver damage. TEM analysis indicated that baicalein inhibited ER stress and triggered autophagy. CCl4-induced elevation of C/EBP homologous protein (CHOP), glucose-regulating protein 78 (GRP78), activating transcription factor 4 (ATF4), activating transcription factor 6 (ATF6), inositol requiring enzyme 1 (IRE1), pancreatic ER kinase (PERK), and active/spliced form of X-box-binding protein 1 (XBP1s) ER stress markers were decreased by baicalein. Baicalein also increased the autophagy-related 5 (ATG5), Beclin1, and Microtubule-associated protein 1A/1B-light chain 3-phosphatidylethanolamine-conjugated form (LC3-II) autophagy marker levels. In conclusion, baicalein reduced the CCl4-induced liver damage by inhibiting ER stress and the trigger of autophagy. |
| Databáze: | OpenAIRE |
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