Gap Junction Formation and Connexin Distribution in Pig Trophoblast before Implantation

Autor: Bernadette Fléchon, F Lefèvre, Jeril Degrouard, J.-E. Fléchon, O Traub
Přispěvatelé: Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2004
Předmět:
Blastomeres
Swine
[SDV]Life Sciences [q-bio]
Blotting
Western
Connexin
Biology
Cell junction
Connexins
Embryonic and Fetal Development
03 medical and health sciences
0302 clinical medicine
Pregnancy
otorhinolaryngologic diseases
medicine
Animals
[INFO]Computer Science [cs]
Embryo Implantation
Blastocyst
Fluorescent Antibody Technique
Indirect
education
ComputingMilieux_MISCELLANEOUS
reproductive and urinary physiology
030304 developmental biology
Genetics
0303 health sciences
education.field_of_study
030219 obstetrics & reproductive medicine
Gap junction
Gap Junctions
Obstetrics and Gynecology
Trophoblast
Blotting
Northern
Blastula
Trophoblasts
Cell biology
Microscopy
Electron
medicine.anatomical_structure
Reproductive Medicine
embryonic structures
Connexin 32
Female
sense organs
IMPLANTATION EMBRYONNAIRE
Endoderm
Cell Division
Developmental Biology
Zdroj: Placenta
Placenta, Elsevier, 2004, 25, pp.85-94
ISSN: 0143-4004
Popis: This study describes the gap junctions in extraembryonic cell layers of the preimplantation pig embryo (trophectoderm and endoderm constituting the trophoblast). Using specific antibodies against connexins 31, 32 and 43, we found these connexins in embryos by immunodetection using Western blot and immunofluorescence analysis. By immunofluorescence, the first foci of connexin 31 were detected in the four-cell stage blastomeres, and the first diffuse gap junctions appeared at the eight-cell stage. Intercellular communication was observed with Lucifer yellow transfer to start also at the eight-cell stage around the onset of compaction. Typical gap junctions developed in the trophectoderm of blastocysts, as observed by transmission electron microscopy of thin sections and freeze-fracture replicas. Connexin proteins were differently expressed in time and space: connexin 31 was continuously present in trophectoderm, connexin 32 was essentially found in endoderm during elongation; connexin 43 was distributed in both trophectoderm and endoderm during blastulation and expansion. Connexin 43 was also found in two isoforms, phosphorylated or not, at day 14. Such developmentally regulated connexin expression may be essentially useful to control the exponential growth of trophoblast in preimplantation pig blastocysts.
Databáze: OpenAIRE
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